Functional Protein Analysis Using Mass Spectrometry

Author(s): Simone Konig.

Journal Name: Current Organic Chemistry

Volume 9 , Issue 9 , 2005

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Mass spectrometry is a very versatile technique which has become both a high-throughput and a research tool in the biosciences. In this review, methods of functional protein analysis are discussed from a perspective in clinical research. The basics of proteomics is introduced as well as the determination of post-translational modifications such as phosphorylation. The investigation of protein interactions involves the measurement of non-covalent complexes and small molecule ligands as well as bioaffinity mass spectrometry. Comparative analysis of sample profiles on specific surfaces is performed increasingly to distinguish disease-related changes.

Keywords: matrix-assisted laser desorption (maldi), ion detector, magnetic sector, capillary electrophoresis, nanospray, phosphorylation analysis, ion microprobe, isotope-ratio mass spectrometry, proteomics, acetylation

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Article Details

Year: 2005
Page: [875 - 887]
Pages: 13
DOI: 10.2174/1385272054038291
Price: $58

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