Screening of Molecular Repertoires by Microbial Surface Display

Author(s): Thomas Jostock, Stefan Dubel.

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 8 , Issue 2 , 2005

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Abstract:

A multitude of systems for the presentation of foreign peptides or proteins on the surface of microorganisms has been developed within the past two decades. However, the majority of the bacterial surface display systems are devoted to the presentation of heterologous antigens to the immune system (vaccine generation). Bacteria are the preferable hosts for the generation of vast genetic repertoires, and their genetic manipulation and cultivation is easy. As a consequence, they provide promising systems for large-scale functional screenings, e.g. for enzyme activity or protein-protein interactions. This review will focus on examples of microbial surface display used for the screening of combinatorial repertoires. Further, we discuss future opportunities and promising candidate proteins not yet employed for that task.

Keywords: epitope mapping, surface display, peptide libraries, combinatorial libraries, antibody selection, mutant selection, gram-positive, gram-negative

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Article Details

VOLUME: 8
ISSUE: 2
Year: 2005
Page: [127 - 133]
Pages: 7
DOI: 10.2174/1386207053258479
Price: $65

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