Abstract
Recent advances in DNA sequencing has made it possible to accurately decipher the entire genetic complement of a probiotic bacterium. Increases in sequencing capabilities have been enhanced through improved computer software that can annotate, or identify, the majority of genes encoded by the sequence. The availability of annotated genome sequence will be important in defining the capabilities of the individual strains of probiotic bacteria. It will also form the platform for microarray and proteomic technologies that allow real-time analysis of RNA and protein expression in the bacterial cell. Investigation of probiotic organisms with these new and potentially powerful tools will facilitate the development of the bacteria as therapeutic agents, and provide the mechanisms to produce advanced probiotic strains. This paper addresses the core technologies in the rapidly growing area of genomics, and their application to the molecular characterisation of probiotic bacteria and host-microbe interactions.
Keywords: genomics, probiotics, lactobacillus, sequencing, annotation, microarrays, proteomics
Current Pharmaceutical Design
Title: Mining the Probiotic Genome: Advanced Strategies, Enhanced Benefits, Perceived Obstacles
Volume: 11 Issue: 1
Author(s): Michael Callanan
Affiliation:
Keywords: genomics, probiotics, lactobacillus, sequencing, annotation, microarrays, proteomics
Abstract: Recent advances in DNA sequencing has made it possible to accurately decipher the entire genetic complement of a probiotic bacterium. Increases in sequencing capabilities have been enhanced through improved computer software that can annotate, or identify, the majority of genes encoded by the sequence. The availability of annotated genome sequence will be important in defining the capabilities of the individual strains of probiotic bacteria. It will also form the platform for microarray and proteomic technologies that allow real-time analysis of RNA and protein expression in the bacterial cell. Investigation of probiotic organisms with these new and potentially powerful tools will facilitate the development of the bacteria as therapeutic agents, and provide the mechanisms to produce advanced probiotic strains. This paper addresses the core technologies in the rapidly growing area of genomics, and their application to the molecular characterisation of probiotic bacteria and host-microbe interactions.
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Cite this article as:
Callanan Michael, Mining the Probiotic Genome: Advanced Strategies, Enhanced Benefits, Perceived Obstacles, Current Pharmaceutical Design 2005; 11 (1) . https://dx.doi.org/10.2174/1381612053382377
DOI https://dx.doi.org/10.2174/1381612053382377 |
Print ISSN 1381-6128 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4286 |
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