Abstract
Transcription factors are a large and diverse group of proteins which bind to DNA response elements in the promoters of genes either activating or repressing gene expression. The purification and characterization of transcription factors has been improving at a rapid pace. New purification techniques, including oligonucleotide trapping, and improved methods of detection, including chromatin immunoprecipitation have been combined with improved characterization using mass spectrometry methods. Here, we review and give examples of many of these recent advances.
Keywords: Reviews, Transcription factors, Purification, Mass spectrometry, Proteomics
Current Analytical Chemistry
Title: Purification of Transcription Factors and Identification with Mass Spectrometry
Volume: 3 Issue: 4
Author(s): Shoulei Jiang, Linda Nagore, Daifeng Jiang, Robert A. Moxley and Harry W. Jarrett
Affiliation:
Keywords: Reviews, Transcription factors, Purification, Mass spectrometry, Proteomics
Abstract: Transcription factors are a large and diverse group of proteins which bind to DNA response elements in the promoters of genes either activating or repressing gene expression. The purification and characterization of transcription factors has been improving at a rapid pace. New purification techniques, including oligonucleotide trapping, and improved methods of detection, including chromatin immunoprecipitation have been combined with improved characterization using mass spectrometry methods. Here, we review and give examples of many of these recent advances.
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Cite this article as:
Jiang Shoulei, Nagore Linda, Jiang Daifeng, Moxley A. Robert and Jarrett W. Harry, Purification of Transcription Factors and Identification with Mass Spectrometry, Current Analytical Chemistry 2007; 3 (4) . https://dx.doi.org/10.2174/157341107782109626
DOI https://dx.doi.org/10.2174/157341107782109626 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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