Screening of Antioxidant Compounds During Sprouting of Brassica oleracea L. var. costata DC

Author(s): Carla Sousa, Graciliana Lopes, David M. Pereira, Marcos Taveira, Patricia Valentao, Rosa M. Seabra, Jose A. Pereira, Paula Baptista, Federico Ferreres, Paula B. Andrade.

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 10 , Issue 5 , 2007

Become EABM
Become Reviewer

Abstract:

The changes in antioxidant compounds of Brassica oleracea L. var. costata DC seeds were monitored during the first twelve days of seedling development. Sprouts were screened at time intervals of two days for phenolic compounds and organic acids. The identified phenolic compounds included esters of sinapic acid with glucose, gentiobiose and kaempferol, as well as sinapoylcholine. The organic acids were oxalic, aconitic, citric, pyruvic, malic, shikimic, and fumaric acids. During germination, a depletion of phenolic compounds was observed, although no qualitative changes were seen. Among individual compounds, kaempferol, choline and glucose esters of sinapic acid showed a marked decrease between days two and six, whereas the changes in gentiobiose esters of sinapic acid were smaller. The total organic acids content increased rapidly during the first four days, with less significant variations thereafter. Malic acid, the major organic acid found in sprouts, greatly contributed to this result though oxalic, pyruvic, and fumaric acids also increased in the same manner. In contrast, aconitic, citric and shikimic acids showed decreases between days two and twelve of germination.

Keywords: Brassica oleracea L. var. costata DC, sprouts, phenolic compounds, organic acids

Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 10
ISSUE: 5
Year: 2007
Page: [377 - 386]
Pages: 10
DOI: 10.2174/138620707781662817
Price: $58

Article Metrics

PDF: 4