Current Pharmaceutical Biotechnology

Davide Prosperi
Department of Biotechnology and Bioscience
University of Milan Bicocca
Milano
Italy

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Bromodeoxyuridine and the Detection of Neurogenesis

Author(s): H. Georg Kuhn, Christiana M. Cooper-Kuhn.

Abstract:

Bromodeoxyuridine (BrdU) is widely used for labeling dividing cells to determine their fate. In particular, the analysis of neurogenesis in the adult mammalian brain has made significant progress through the use of this technique. However; when using BrdU for labeling, there are several issues to consider in order to minimalize possible cytotoxicity or false-positive labeling. This current review summarizes methodological and technical aspects of BrdU administration and detection, compares alternative methods and gives recommendations on how to avoid labeling artifacts.

Keywords: Neurogenesis, proliferation, bromodeoxyuridine, brain, cell death, differentiation, detection methods

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Article Details

VOLUME: 8
ISSUE: 3
Year: 2007
Page: [127 - 131]
Pages: 5
DOI: 10.2174/138920107780906531
Price: $58