Abstract
Most proteins required for chloroplast function are encoded in the nuclear genome and have to be translocated into the organelle upon synthesis on cytosolic ribosomes. The translocation is facilitated by a proteinaceous machinery located in the outer and inner chloroplast membrane, the Toc and Tic complexes (translocon at the outer/inner chloroplast membrane). In the past years, many components of these complexes -including receptors, channels and regulatory proteins - have been isolated and characterized biochemically. Recently, the functional analysis of these proteins was complemented by characterization of corresponding loss of function mutants in Arabidopsis thaliana as a model system. Here, we will discuss these in vivo data and the results of expression profiling in the context of current biochemical models.
Keywords: Protein import, Toc components, Tic components, knock out analysis, Arabidopsis thaliana
Current Genomics
Title: Chloroplast Protein Import: Reverse Genetic Approaches
Volume: 7 Issue: 4
Author(s): Mislav Oreb, Katharina Reger and Enrico Schleiff
Affiliation:
Keywords: Protein import, Toc components, Tic components, knock out analysis, Arabidopsis thaliana
Abstract: Most proteins required for chloroplast function are encoded in the nuclear genome and have to be translocated into the organelle upon synthesis on cytosolic ribosomes. The translocation is facilitated by a proteinaceous machinery located in the outer and inner chloroplast membrane, the Toc and Tic complexes (translocon at the outer/inner chloroplast membrane). In the past years, many components of these complexes -including receptors, channels and regulatory proteins - have been isolated and characterized biochemically. Recently, the functional analysis of these proteins was complemented by characterization of corresponding loss of function mutants in Arabidopsis thaliana as a model system. Here, we will discuss these in vivo data and the results of expression profiling in the context of current biochemical models.
Export Options
About this article
Cite this article as:
Oreb Mislav, Reger Katharina and Schleiff Enrico, Chloroplast Protein Import: Reverse Genetic Approaches, Current Genomics 2006; 7 (4) . https://dx.doi.org/10.2174/138920206778427004
DOI https://dx.doi.org/10.2174/138920206778427004 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
Call for Papers in Thematic Issues
Advanced AI Techniques in Big Genomic Data Analysis
The thematic issue on "Advanced AI Techniques in Big Genomic Data Analysis" aims to explore the cutting-edge methodologies and applications of artificial intelligence (AI) in the realm of genomic research, where vast amounts of data pose both challenges and opportunities. This issue will cover a broad spectrum of AI-driven strategies, ...read more
Advanced Computational Algorithms and Artificial Intelligence in Clinical Pharmacogenomics
In the era of personalized medicine, understanding the relationship between genetics and drug response is crucial. This issue delves into innovative methodologies, leveraging deep computational analysis and artificial intelligence, to enhance the field of Clinical Pharmacogenomics. The interdisciplinary approach harnesses the power of advanced high-throughput genotyping technologies, sophisticated computational analysis, ...read more
Applications of Single-cell Sequencing Technology in Reproductive Medicine
Single cell sequencing (SCS) technology utilizes individual cells' genetic material to sequence their genome, transcriptome, and epigenetics at the molecular level. It offers insights into cell heterogeneity and enables the study of limited biological materials. Since its recognition as a valuable technique in 2011, single cell sequencing has yielded numerous ...read more
Big Data in Cancer Research
Cancer is a significant threat to human life and health, remaining a highly aggressive killer. It is a leading cause of death worldwide and represents a crucial medical issue for humanity. However, in the past decade, the effectiveness of new synthetic anticancer agents has not matched the current clinical speculation. ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Sleep Disordered Breathing: Alternatives to CPAP Therapy
Current Respiratory Medicine Reviews PiWi RNA in Neurodevelopment and Neurodegenerative Disorders
Current Molecular Pharmacology Genetics and Genomics Interventions for Promoting Millets as Functional Foods
Current Genomics Roles of Hematopoietic Stem and Progenitor Cells in Ischemic Cardiovascular Disease
Current Stem Cell Research & Therapy Current Status and Future Prospects of C1 Domain Ligands as Drug Candidates
Current Topics in Medicinal Chemistry Sphingosine Kinases Signalling in Carcinogenesis
Mini-Reviews in Medicinal Chemistry Anabolics in Osteoporosis: The Emerging Therapeutic Tool
Current Molecular Medicine TGFb and its Smad Connection to Cancer
Current Genomics Micro-/Nano-Scale Biointerfaces, Mechanical Coupling and Cancer Therapy
Current Topics in Medicinal Chemistry Endogenous Ligand Bias by Chemokines: Implications at the Front Lines of Infection and Leukocyte Trafficking
Endocrine, Metabolic & Immune Disorders - Drug Targets microRNA Decay: Refining microRNA Regulatory Activity
MicroRNA Importance of Oxidative Damage on the Electron Transport Chain for the Rational Use of Mitochondria-Targeted Antioxidants
Mini-Reviews in Medicinal Chemistry Cosignaling Molecules Around LIGHT-HVEM-BTLA: From Immune Activation to Therapeutic Targeting
Current Molecular Medicine Breaking the DNA Damage Response via Serine/Threonine Kinase Inhibitors to Improve Cancer Treatment
Current Medicinal Chemistry Platelet Activation by Extracellular Matrix Proteins in Haemostasis and Thrombosis
Current Pharmaceutical Design Combining Angiogenesis Inhibitors with Radiation: Advances and Challenges in Cancer Treatment
Current Pharmaceutical Design Growth Factors in the Pathogenesis of Retinal Neurodegeneration in Diabetes Mellitus
Current Neuropharmacology Treatment of Obesity: Should We Target the Individual or Society?
Current Pharmaceutical Design Use of Medications for the Treatment of Alcohol Dependence: A Retrospective Study Conducted in 2011-2012
Current Drug Research Reviews Biochemical and Histochemical Comparison of Cholinesterases in Normal and Alzheimer Brain Tissues
Current Alzheimer Research