Crystallization and Preliminary Crystallographic Analysis of Allograft Inflammatory Factor 1

Author(s): Shentao Li, Xiaoping Liao, Zhangliu Chen, Mark Bartlam.

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 8 , 2006

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Abstract:

Allograft inflammatory factor-1 (AIF-1) is a 17-kDa IFN- γ inducible Ca2+-binding EF-hand protein involved in immune dysfunction and smooth muscle cell activation. AIF-1 was solubly expressed in E. coli and purified. Crystals of AIF-1 were grown at 291 K using PEG-8000 as precipitant. Diffraction by the AIF-1 crystal extends to 3.3 Å resolution, and the crystal belongs to the space group P43 with unit cell parameters a=b=73.4, c=49.1 Å .

Keywords: Allograft inflammatory factor 1, prokaryotic expression, crystallization

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Article Details

VOLUME: 13
ISSUE: 8
Year: 2006
Page: [855 - 858]
Pages: 4
DOI: 10.2174/092986606777841208
Price: $58