Abstract
Exendin-4 is a 39 amino acid peptide isolated from the Gila monster salivary gland. It is 53% homologous to GLP-1 and exhibits similar glucoregulatory activities. In this study, exendin-4 dimer (D-Ex4) was constructed, cloned into plasmid pET32a(+) and expressed in E. coli BL21(DE3). The fusion protein with His-tag at the N-terminus was purified with a Ni-NTA-agarose column. After proteolytic cleavage, D-Ex4 peptide with high purity was obtained by HPLC. The results obtained by chemical cross-linking showed that D-Ex4 maintained affinity to GLP-1 receptor.
Keywords: Exendin-4 dimer, recombinant expression, purification, chemical cross-linking
Protein & Peptide Letters
Title: Expression and Purification of Exendin-4 Dimer in Escherichia coli and Its Interaction with GLP-1 Receptor In Vitro
Volume: 13 Issue: 8
Author(s): Lina Yi, Xiaopu Yin, Dongzhi Wei and Yushu Ma
Affiliation:
Keywords: Exendin-4 dimer, recombinant expression, purification, chemical cross-linking
Abstract: Exendin-4 is a 39 amino acid peptide isolated from the Gila monster salivary gland. It is 53% homologous to GLP-1 and exhibits similar glucoregulatory activities. In this study, exendin-4 dimer (D-Ex4) was constructed, cloned into plasmid pET32a(+) and expressed in E. coli BL21(DE3). The fusion protein with His-tag at the N-terminus was purified with a Ni-NTA-agarose column. After proteolytic cleavage, D-Ex4 peptide with high purity was obtained by HPLC. The results obtained by chemical cross-linking showed that D-Ex4 maintained affinity to GLP-1 receptor.
Export Options
About this article
Cite this article as:
Yi Lina, Yin Xiaopu, Wei Dongzhi and Ma Yushu, Expression and Purification of Exendin-4 Dimer in Escherichia coli and Its Interaction with GLP-1 Receptor In Vitro, Protein & Peptide Letters 2006; 13 (8) . https://dx.doi.org/10.2174/092986606777841226
DOI https://dx.doi.org/10.2174/092986606777841226 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Pathophysiological Features of Obesity and its Impact on Cognition: Exercise Training as a Non-Pharmacological Approach
Current Pharmaceutical Design Role of Bioactive Phytoconstituents as Modulators of Hepatic Carbohydrates Metabolising Enzymes: A Target Specific Approach to Treat Diabetes Mellitus
Current Diabetes Reviews Mass Spectrometry in the Study of Hemoglobin: from Covalent Structure to Higher Order Assembly
Current Organic Chemistry Targeting Insulin Amyloid Assembly by Aminosugars and Their Derivatives
Protein & Peptide Letters Psychosocial Risk Factors Related to Ischemic Heart Disease in Women
Current Pharmaceutical Design Recent Updates of Chemically Modified Electrodes in Pharmaceutical Analysis
Combinatorial Chemistry & High Throughput Screening Processing of Amyloid Precursor Protein as a Biochemical Link Between Atherosclerosis and Alzheimers Disease
Cardiovascular & Hematological Disorders-Drug Targets The p35 Family of Apoptosis Inhibitors
Current Genomics Imidazoline Binding Sites in the Endocrine Pancreas: Can They Fulfil Their Potential as Targets for the Development of New Insulin Secretagogues?
Current Pharmaceutical Design Proteome Adjustments Post Gradual Hypoxic Stress in Barilius bendelisis: Insights into Adaptive Strategies of a Hill Stream Cyprinid
Current Proteomics Role of Oxidative Stress in the Genesis of Atherosclerosis and Diabetes Mellitus: A Personal Look Back on 50 Years of Research
Current Aging Science Modulation of MicroRNAs by <i>Euphorbia Microsciadia</i> Boiss in MDA-MB-231 Cell Line: New Possibilities in Breast Cancer Therapy
Recent Patents on Anti-Cancer Drug Discovery Association of Val16Ala Polymorphism of Manganese Superoxide Dismutase (MnSOD) with Food Intake and Cardiometabolic Risk Factors in the Elderly in Primary Care in Porto Alegre
Current Aging Science Identification of Novel β3-Adrenoceptor Agonists Using Energetic Analysis, Structure Based Pharmacophores and Virtual Screening
Combinatorial Chemistry & High Throughput Screening Mechanisms of Immune Modulation by Mesenchymal Stromal Cells and Clinical Translation
Current Molecular Medicine Reproductive Health Indicators and Factors Affecting Menstrual Cycle Pattern among Sudanese Women with Thyroid Dysfunction
Current Women`s Health Reviews Gastrointestinal Region Specific Insulin Permeation Enhancement by <i>Aloe vera</i> Gel
Drug Delivery Letters Inhaled Insulin and the Lung
Current Medicinal Chemistry Virtual Screening of NCI Diversity Set II Lead to New Cyclin-Dependent Kinases 5 Inhibitors Using AutoDock
Letters in Drug Design & Discovery T-wave Area Dispersion in Coronary Artery Bypass Grafting is Indicative for Increased Risk of Adverse Events in Diabetics
Current Diabetes Reviews