Abstract
Ribonuclease inhibitor (RI), a 50 kDa protein, has been found both in mammalian and nonmammalian tissues. We have isolated RI from goat liver and partial characterization has been accomplished. For the isolation of RI, DEAE cellulose column chromatography followed by affinity chromatography using CNBr activated Sepharose 4B was performed. The inhibition of ribonucleolytic activity of Ribonuclease A has been checked by an agarose gel based assay. The antiangiogenic property of the protein was tested by the chorioallantoic membrane (CAM) assay. Results indicate inhibition of angiogenesis.
Keywords: Ribonuclease inhibitor, agarose gel based assay, angiogenesis, CAM assay
Protein & Peptide Letters
Title: Isolation and Partial Characterization of Ribonuclease Inhibitor from Goat Liver
Volume: 13 Issue: 8
Author(s): Juin Chatterjee, Tushar Kanti Maiti and Swagata Dasgupta
Affiliation:
Keywords: Ribonuclease inhibitor, agarose gel based assay, angiogenesis, CAM assay
Abstract: Ribonuclease inhibitor (RI), a 50 kDa protein, has been found both in mammalian and nonmammalian tissues. We have isolated RI from goat liver and partial characterization has been accomplished. For the isolation of RI, DEAE cellulose column chromatography followed by affinity chromatography using CNBr activated Sepharose 4B was performed. The inhibition of ribonucleolytic activity of Ribonuclease A has been checked by an agarose gel based assay. The antiangiogenic property of the protein was tested by the chorioallantoic membrane (CAM) assay. Results indicate inhibition of angiogenesis.
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Cite this article as:
Chatterjee Juin, Kanti Maiti Tushar and Dasgupta Swagata, Isolation and Partial Characterization of Ribonuclease Inhibitor from Goat Liver, Protein & Peptide Letters 2006; 13 (8) . https://dx.doi.org/10.2174/092986606777841307
DOI https://dx.doi.org/10.2174/092986606777841307 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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