Isolation and Partial Characterization of Ribonuclease Inhibitor from Goat Liver

Author(s): Juin Chatterjee, Tushar Kanti Maiti, Swagata Dasgupta.

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 8 , 2006

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Abstract:

Ribonuclease inhibitor (RI), a 50 kDa protein, has been found both in mammalian and nonmammalian tissues. We have isolated RI from goat liver and partial characterization has been accomplished. For the isolation of RI, DEAE cellulose column chromatography followed by affinity chromatography using CNBr activated Sepharose 4B was performed. The inhibition of ribonucleolytic activity of Ribonuclease A has been checked by an agarose gel based assay. The antiangiogenic property of the protein was tested by the chorioallantoic membrane (CAM) assay. Results indicate inhibition of angiogenesis.

Keywords: Ribonuclease inhibitor, agarose gel based assay, angiogenesis, CAM assay

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Article Details

VOLUME: 13
ISSUE: 8
Year: 2006
Page: [779 - 783]
Pages: 5
DOI: 10.2174/092986606777841307
Price: $58