Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of the Human Intracellular Chloride Channel Protein CLIC4

Author(s): De-Feng Li, Yun-Feng Li, Qiu-Hua Huang, Ying Zhang, Da-Cheng Wang.

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 6 , 2006

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The human chloride intracellular channel protein CLIC4 has been crystallized by the hanging-drop vapourdiffusion technique using trisodium citrate as the precipitant. The best crystals were obtained by the microseeding method. The crystals diffracted to 2.2 Å resolution and were found to belong to space group P121, with unit-cell parameters a = 73.19, b =86.05, c = 73.38 Å, β = 112.99° and three molecule per asymmetric unit.

Keywords: CLIC4, human chloride intracellular channel protein, purification, crystallization, preliminary crystallographic studies

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Article Details

Year: 2006
Page: [641 - 643]
Pages: 3
DOI: 10.2174/092986606777145742
Price: $58

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