Abstract
The human chloride intracellular channel protein CLIC4 has been crystallized by the hanging-drop vapourdiffusion technique using trisodium citrate as the precipitant. The best crystals were obtained by the microseeding method. The crystals diffracted to 2.2 Å resolution and were found to belong to space group P121, with unit-cell parameters a = 73.19, b =86.05, c = 73.38 Å, β = 112.99° and three molecule per asymmetric unit.
Keywords: CLIC4, human chloride intracellular channel protein, purification, crystallization, preliminary crystallographic studies
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