Crystallization and Preliminary X-Ray Analysis of Sau3AI C-Terminal 232-419 Amino Acids Fragment

Author(s): Feng Yu, Jiaping Song, Chunyan Xu, Yu Ding, Xiaojian Hu, Jianhua He, Zhihong Zhang.

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 6 , 2006

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The C-terminal 232-419 amino acids fragment of endonuclease Sau3AI has been successfully expressed in Escherichia coli with 6 His at its N-terminal. After purification and crystallization, one completed 2.8 Å data set was collected using a Rigaku R-AXIS IV ++ diffractometer. The plate-like crystals belong to orthorhombic space group P212121 with the cell dimension of a = 34.75, b = 76.82, c = 123.59Å and contain one molecule per asymmetric unit.

Keywords: Sau3AI endonuclease, crystallization, preliminary X-ray analysis

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Article Details

Year: 2006
Page: [627 - 628]
Pages: 2
DOI: 10.2174/092986606777145724
Price: $58

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