Crystallization and Preliminary X-Ray Analysis of Sau3AI C-Terminal 232-419 Amino Acids Fragment

Title: Crystallization and Preliminary X-Ray Analysis of Sau3AI C-Terminal 232-419 Amino Acids Fragment

Volume: 13 Issue: 6

Author(s): Feng Yu, Jiaping Song, Chunyan Xu, Yu Ding, Xiaojian Hu, Jianhua He and Zhihong Zhang

Affiliation:

Keywords: Sau3AI endonuclease, crystallization, preliminary X-ray analysis

Abstract: The C-terminal 232-419 amino acids fragment of endonuclease Sau3AI has been successfully expressed in Escherichia coli with 6 His at its N-terminal. After purification and crystallization, one completed 2.8 Å data set was collected using a Rigaku R-AXIS IV ++ diffractometer. The plate-like crystals belong to orthorhombic space group P212121 with the cell dimension of a = 34.75, b = 76.82, c = 123.59Å and contain one molecule per asymmetric unit.

Cite this article as:

Yu Feng, Song Jiaping, Xu Chunyan, Ding Yu, Hu Xiaojian, He Jianhua and Zhang Zhihong, Crystallization and Preliminary X-Ray Analysis of Sau3AI C-Terminal 232-419 Amino Acids Fragment, Protein & Peptide Letters 2006; 13 (6) . https://dx.doi.org/10.2174/092986606777145724