cDNA Cloning, Purification and Properties of Paralithodes camtschatica Metalloprotease

Author(s): Svetlana A. Semenova, Galina N. Rudenskaya, Denis V. Rebrikov, Vyacheslav A. Isaev.

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 6 , 2006

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Abstract:

Hepatopancreas of king crab Paralithodes camtschatica produces a metalloprotease, which belongs to the astacin family, as cDNA cloning and sequencing showed. The metalloprotease has been purified chromatographically to apparent homogeneity. The purification factor was 16 and activity recovery was 20%. pH and temperature optimum have been determined. In its properties (molecular weight, pI, metal content) the metalloprotease is close to crayfish astacin. However, analysis of the enzyme sequences revealed differences, which account for differences in substrate specificities and imply a different activation mechanism.

Keywords: Metalloprotease, astacin family, affinity chromatography, Paralithodes camtschatica

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Article Details

VOLUME: 13
ISSUE: 6
Year: 2006
Page: [571 - 575]
Pages: 5
DOI: 10.2174/092986606777145887
Price: $58

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