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Protein & Peptide Letters
ISSN (Print): 0929-8665
ISSN (Online): 1875-5305
VOLUME: 13
ISSUE: 5
DOI: 10.2174/092986606776819637      Price:  $58









Important Contributions of a New Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) Procedure for Elucidating Metal Cofactor Metabolisms in Protein-Misfolding Diseases - A Theory

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Author(s): Bernd Kastenholz
Pages 503-508 (6)
Abstract:
The quantitative analysis of metallochaperone proteins in biofluids (e.g. blood, liquor) may be a major prerequisite for clinical investigations concerning the structure-function relationships of biologically-active metal cofactorcontaining chaperones in protein-misfolding diseases (e.g. Alzheimers or related diseases). For these purposes, a new state-of-the-art gel electrophoresis [quantitative preparative native continuous polyacrylamide gel electrophoresis procedure (QPNC-PAGE)] combined with biological mass and NMR spectrometries might essentially contribute to provide fundamental insights into the metabolisms of important metal cofactors in biological systems and the proper folding of metallochaperones in conformational diseases.
Keywords:
QPNC-PAGE, GPC, NMR, ICP-MS, Quantitative analysis, Biologically-active metallochaperones, Metal cofactorcontaining proteins, Native conformation, Chemical stability, Alzheimer's disease
Affiliation:
Institute for Chemistryand Dynamics of the Geosphere, Institute III: Phytosphere, ResearchCentre Juelich, Juelich 52425, Germany.