Crystallization and Preliminary X-Ray Crystallographic Analysis of Yeast Tyrosyl-tRNA Synthetase Complexed with Its Cognate tRNA
Yoshio Kusakabe, Satoshi Ohno, Nobutada Tanaka, Masashi Nakamura, Masaru Tsunoda, Tomohisa Moriguchi, Norio Asai, Mitsuo Sekine, Takashi Yokogawa, Kazuya Nishikawa and Kazuo T. Nakamura
Affiliation: School of PharmaceuticalSciences, Showa University, Tokyo 142-8555, Japan.
Keywords: aminoacyl-tRNA synthetase, crystallization, tRNA, TyrRS
Yeast tyrosyl-tRNA synthetase (yTyrRS) has been crystallized by the vapor diffusion method in the presence of its cognate tRNATyr. The crystals belong to a tetragonal space group P41212 with cell dimensions of a = b = 63.85 Å, and c = 330.3 Å. The asymmetric unit contains one molecule each of yTyrRS and tRNATyr (one-half of a 2:2 complex). Xray diffraction data have been collected up to 2.5 Å resolution.
Rights & PermissionsPrintExport