Protein & Peptide Letters

Prof. Ben M. Dunn  
Department of Biochemistry and Molecular Biology
University of Florida
College of Medicine
P.O. Box 100245
Gainesville, FL


Crystallization and Preliminary X-Ray Crystallographic Analysis of Yeast Tyrosyl-tRNA Synthetase Complexed with Its Cognate tRNA

Author(s): Yoshio Kusakabe, Satoshi Ohno, Nobutada Tanaka, Masashi Nakamura, Masaru Tsunoda, Tomohisa Moriguchi, Norio Asai, Mitsuo Sekine, Takashi Yokogawa, Kazuya Nishikawa and Kazuo T. Nakamura

Affiliation: School of PharmaceuticalSciences, Showa University, Tokyo 142-8555, Japan.

Keywords: aminoacyl-tRNA synthetase, crystallization, tRNA, TyrRS


Yeast tyrosyl-tRNA synthetase (yTyrRS) has been crystallized by the vapor diffusion method in the presence of its cognate tRNATyr. The crystals belong to a tetragonal space group P41212 with cell dimensions of a = b = 63.85 Å, and c = 330.3 Å. The asymmetric unit contains one molecule each of yTyrRS and tRNATyr (one-half of a 2:2 complex). Xray diffraction data have been collected up to 2.5 Å resolution.

Order Reprints Order Eprints Rights & PermissionsPrintExport

Article Details

Page: [417 - 419]
Pages: 3
DOI: 10.2174/092986606775974465