The Early Events of α-Synuclein Oligomerization Revealed by Photo- Induced Cross-Linking

Author(s): Hong-Tao Li, Xiao-Jing Lin, Yuan-Yuan Xie, Hong-Yu Hu.

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 4 , 2006

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Abstract:

Assembly of α-synuclein (α-Syn) into neurotoxic oligomers and fibrils is an important pathogenic feature of Parkinsons disease. Studying the early events of α-Syn aggregation, such as oligomerization and nucleation, is indispensable to understanding the complicated process. Here, photo-induced cross-linking of unmodified proteins (PICUP) technique is applied to elucidate the early-stage oligomerization of α-Syn. Results show that α-Syn in solution exhibits a mixture of various species, including at least monomers, dimers and trimers. Aggregation of α-Syn probably originates from the dimeric and trimeric seeds. Furthermore, the N-terminal amphipathic region is proposed to be required for the oligomerization (dimerization and trimerization) process. This observation may extend our knowledge on the early events of α-Syn aggregation and the neurotoxic aggregation species.

Keywords: α-synuclein, oligomerization, PICUP, dimmer, trimer

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Article Details

VOLUME: 13
ISSUE: 4
Year: 2006
Page: [385 - 390]
Pages: 6
DOI: 10.2174/092986606775974384
Price: $58

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