Abstract
Assembly of α-synuclein (α-Syn) into neurotoxic oligomers and fibrils is an important pathogenic feature of Parkinsons disease. Studying the early events of α-Syn aggregation, such as oligomerization and nucleation, is indispensable to understanding the complicated process. Here, photo-induced cross-linking of unmodified proteins (PICUP) technique is applied to elucidate the early-stage oligomerization of α-Syn. Results show that α-Syn in solution exhibits a mixture of various species, including at least monomers, dimers and trimers. Aggregation of α-Syn probably originates from the dimeric and trimeric seeds. Furthermore, the N-terminal amphipathic region is proposed to be required for the oligomerization (dimerization and trimerization) process. This observation may extend our knowledge on the early events of α-Syn aggregation and the neurotoxic aggregation species.
Keywords: α-synuclein, oligomerization, PICUP, dimmer, trimer
Protein & Peptide Letters
Title: The Early Events of α-Synuclein Oligomerization Revealed by Photo- Induced Cross-Linking
Volume: 13 Issue: 4
Author(s): Hong-Tao Li, Xiao-Jing Lin, Yuan-Yuan Xie and Hong-Yu Hu
Affiliation:
Keywords: α-synuclein, oligomerization, PICUP, dimmer, trimer
Abstract: Assembly of α-synuclein (α-Syn) into neurotoxic oligomers and fibrils is an important pathogenic feature of Parkinsons disease. Studying the early events of α-Syn aggregation, such as oligomerization and nucleation, is indispensable to understanding the complicated process. Here, photo-induced cross-linking of unmodified proteins (PICUP) technique is applied to elucidate the early-stage oligomerization of α-Syn. Results show that α-Syn in solution exhibits a mixture of various species, including at least monomers, dimers and trimers. Aggregation of α-Syn probably originates from the dimeric and trimeric seeds. Furthermore, the N-terminal amphipathic region is proposed to be required for the oligomerization (dimerization and trimerization) process. This observation may extend our knowledge on the early events of α-Syn aggregation and the neurotoxic aggregation species.
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Cite this article as:
Li Hong-Tao, Lin Xiao-Jing, Xie Yuan-Yuan and Hu Hong-Yu, The Early Events of α-Synuclein Oligomerization Revealed by Photo- Induced Cross-Linking, Protein & Peptide Letters 2006; 13 (4) . https://dx.doi.org/10.2174/092986606775974384
DOI https://dx.doi.org/10.2174/092986606775974384 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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