Protein & Peptide Letters

Prof. Ben M. Dunn  
Department of Biochemistry and Molecular Biology
University of Florida
College of Medicine
P.O. Box 100245
Gainesville, FL
USA
Email: bdunn@ufl.edu

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Production and Purification of Recombinant Human Glucagon Overexpressed as Intein Fusion Protein in Escherichia coli

Author(s): Roman S. Esipov, Vasily N. Stepanenko, Alexandr I. Gurevich, Larisa A. Chupova and Anatoly I. Miroshnikov

Affiliation: Shemyakin-OvchinnikovInstitute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997 Russia.

Keywords: Glucagon, mini-intein, autocatalitic cleavage, recombinant, polypeptide

Abstract:

Chemico-enzymatic synthesis and cloning in Esherichia coli of an artificial gene coding human glucagon was performed. Recombinant plasmid containing hybrid glucagons gene and intein Ssp dnaB from Synechocestis sp. was designed. Expression of the obtained hybrid gene in E. coli, properties of the formed hybrid protein, and conditions of its autocatalytic cleavage leading to glucagon formation were studied.

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Article Details

VOLUME: 13
ISSUE: 4
Page: [343 - 347]
Pages: 5
DOI: 10.2174/092986606775974320