Production and Purification of Recombinant Human Glucagon Overexpressed as Intein Fusion Protein in Escherichia coli

Author(s): Roman S. Esipov, Vasily N. Stepanenko, Alexandr I. Gurevich, Larisa A. Chupova, Anatoly I. Miroshnikov.

Journal Name:Protein & Peptide Letters

Volume 13 , Issue 4 , 2006

Abstract:

Chemico-enzymatic synthesis and cloning in Esherichia coli of an artificial gene coding human glucagon was performed. Recombinant plasmid containing hybrid glucagons gene and intein Ssp dnaB from Synechocestis sp. was designed. Expression of the obtained hybrid gene in E. coli, properties of the formed hybrid protein, and conditions of its autocatalytic cleavage leading to glucagon formation were studied.

Keywords: Glucagon, mini-intein, autocatalitic cleavage, recombinant, polypeptide

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Article Details

VOLUME: 13
ISSUE: 4
Year: 2006
Page: [343 - 347]
Pages: 5
DOI: 10.2174/092986606775974320
Price: $58