Quasielastic Light Scattering Study of Amyloid β-Protein Fibril Formation
Aleksey Lomakin and David B. Teplow
Affiliation: Department of Physics, MIT,77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Keywords: Alzheimer's disease, amyloid β-protein, aggregation, fibril formation, quasielastic light scattering, regularization, elongation, nucleation
Quasielastic light scattering spectroscopy (QLS) is an optical method for the determination of diffusion coefficients of particles in solution. Here we discuss the principles of QLS and explain how the distribution of particle sizes can be reconstructed from the measured correlation function of scattered light. Non-invasive observation of the temporal evolution of particle sizes provides a powerful tool for studying protein assembly. We illustrate practical applications of QLS with examples from studies of fibril formation of the amyloid β-protein.
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