Expression, Purification, Crystallization and Preliminary X-Ray Analysis of Human Spindlin1, an Ovarian Cancer-Related Protein

Author(s): Fuguo Jiang , Qiang Zhao , Lipeng Qin , Hai Pang , Xuetao Pei , Zihe Rao .

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 2 , 2006

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Abstract:

Human spindlin1 is A newly screened and identified gene product related to ovarian carcinomas and is highly homologous to mouse spindlin. It is an abundant maternal transcript expressed in the mouse during the transition from oocyte to embryo. Here, the recombinant human spindlin1 has been overexpressed in Escherichia coli BL21, purified and crystallized using the hanging-drop vapour-diffusion method. Crystals diffracting to 2.25 Å resolution were obtained using ammonium sulfate as precipitant. The crystals belong to the space group P212121, with unit-cell parameters A =40.7 Å, b =84.4 Å, c =136.4 Å, α=β=γ=90° . Assuming two molecules per asymmetric unit, the solvent content is calculated to be 42.4%.

Keywords: gametes, Y-linked spermiogenesis-specific transcript, c-mos, tumorigenesis, Escherichia coli strain BL21, Crystallization

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Article Details

VOLUME: 13
ISSUE: 2
Year: 2006
Page: [203 - 205]
Pages: 3
DOI: 10.2174/092986606775101661
Price: $58

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