Use of Nano-Electrospray for Metabolite Identification and QuantitativeAbsorption, Distribution, Metabolism and Excretion Studies

Author(s): Cornelis E.C.A. Hop.

Journal Name: Current Drug Metabolism

Volume 7 , Issue 5 , 2006

Abstract:

Determination of the pharmacokinetics and metabolite identification have been an integral part of drug discov-ery and development to ensure that drugs have appropriate absorption, distribution, metabolism and excretion properties.Liquid chromatography interfaced with a mass spectrometer has greatly facilitated these studies. Nano-electrospray hasdistinct sensitivity advantages and the increased amount of time available to perform mass spectrometric experiments fa-cilitates structural characterization of metabolites. The recently developed silicon chip-based nano-electrospray devicesare more practical than pulled capillaries. The use of these devices for the determination of pharmacokinetics and metabo-lite identification will be described and particular attention will be paid to the distinct advantages and disadvantages thesedevices offer.

Keywords: ADME, LC-MS/MS, metabolite identification, nano-electrospray, drug metabolism

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Article Details

VOLUME: 7
ISSUE: 5
Year: 2006
Page: [557 - 563]
Pages: 7
DOI: 10.2174/138920006777697909
Price: $58

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