Delivery Strategies for siRNA-mediated Gene Silencing
Ian R. Gilmore, Stephen P. Fox, Andrew J. Hollins and Saghir Akhtar
Pages 147-155 (9)
RNA interference (RNAi) represents a promising new gene silencing technology for functional genomics and a potential therapeutic strategy for a variety of genetic diseases. RNAi involves the targeted post-transcriptional degradation of messenger RNA thereby inhibiting the synthesis of the desired protein. This effectively leads to silencing of gene expression. The effectors of this process are short interfering RNA (siRNA) duplexes (∼21-23nt) that are key intermediaries in the specific degradation of target mRNA following incorporation into the RNA-induced silencing complex (RISC) in the cytosol. However, due to the large molecular weight and negative charge of siRNA duplexes the effective cellular uptake and intracellular delivery appear to represent a major challenge for the widespread use of RNAi in vivo. This review summarises some of the main delivery strategies that have been attempted for the transfection of siRNA to cells in vitro and in vivo.
Short interfering RNA, design, delivery, RNA interference, RNAi, antisense oligonucleotides, transfection, gene therapy
Centre for Genome-basedTherapeutics, The Welsh School of Pharmacy, Cardiff University, RedwoodBuilding, King Edward VII Avenue, Cardiff, CF10 3XF, UK.