Protein & Peptide Letters

Prof. Ben M. Dunn  
Department of Biochemistry and Molecular Biology
University of Florida
College of Medicine
P.O. Box 100245
Gainesville, FL
USA
Email: bdunn@ufl.edu

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Oxidative Folding of Conotoxins in Immobilized Systems

Author(s): B. R. Green and G. Bulaj

Affiliation: Cognetix, Inc., 421 WakaraWay, Suite 201, Salt Lake City, Utah 84108, USA.

Abstract:

We tested two alternative oxidation strategies to produce conotoxins α-GI and μ-PIIIA. The peptides were either reversibly immobilized on a solid support and then oxidized, or the immobilized disulfide reagent (CLEAR-OX™) was used to oxidize the peptides. Both strategies appeared more efficient at higher peptide concentrations, consistent with pseudo-dilution effects.

Keywords: Conotoxins, Oxidative folding, Immobilized, CLEAR-OXTM

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Article Details

VOLUME: 13
ISSUE: 1
Page: [67 - 70]
Pages: 4
DOI: 10.2174/092986606774502162