Oxidative Folding of Conotoxins in Immobilized Systems
B. R. Green and G. Bulaj
Affiliation: Cognetix, Inc., 421 WakaraWay, Suite 201, Salt Lake City, Utah 84108, USA.
We tested two alternative oxidation strategies to produce conotoxins α-GI and μ-PIIIA. The peptides were either reversibly immobilized on a solid support and then oxidized, or the immobilized disulfide reagent (CLEAR-OX™) was used to oxidize the peptides. Both strategies appeared more efficient at higher peptide concentrations, consistent with pseudo-dilution effects.
Keywords: Conotoxins, Oxidative folding, Immobilized, CLEAR-OXTM
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