Abstract
As predicted, in the post-genomic era microarray technology has a major impact on our understanding of complex gene expression patterns and circuit function in the brain. We increasingly appreciate that, due to the phenotypic and transcript complexity, brain transcriptome profiling data are multifaceted and are best interpreted in the context of the cellular diversity of the studied brain region. However, despite advances made over the past five years, biological interpretation of massive microarray datasets remains a significant challenge. Although we are becoming more efficient in separating "true" transcriptome differences from experimental noise, verification of microarray data and anatomical localization of expression changes to neuronal subpopulations will continue to be an integral part of brain microarray experiments.
Keywords: neurons, hybridization, NR3A transcript, RT-PCR, gene expression
Current Genomics
Title: Microarrays in Brain Research: Data Quality and Limitations Revisited
Volume: 7 Issue: 1
Author(s): Karoly Mirnics
Affiliation:
Keywords: neurons, hybridization, NR3A transcript, RT-PCR, gene expression
Abstract: As predicted, in the post-genomic era microarray technology has a major impact on our understanding of complex gene expression patterns and circuit function in the brain. We increasingly appreciate that, due to the phenotypic and transcript complexity, brain transcriptome profiling data are multifaceted and are best interpreted in the context of the cellular diversity of the studied brain region. However, despite advances made over the past five years, biological interpretation of massive microarray datasets remains a significant challenge. Although we are becoming more efficient in separating "true" transcriptome differences from experimental noise, verification of microarray data and anatomical localization of expression changes to neuronal subpopulations will continue to be an integral part of brain microarray experiments.
Export Options
About this article
Cite this article as:
Mirnics Karoly, Microarrays in Brain Research: Data Quality and Limitations Revisited, Current Genomics 2006; 7 (1) . https://dx.doi.org/10.2174/138920206776389739
DOI https://dx.doi.org/10.2174/138920206776389739 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Multicomponent Synthesis of Novel Class of Isatin and 5-isatinyli-denerhodanine Based Diaminofuran Derivatives
Letters in Organic Chemistry LC/MS/MS Profiling of Tissue Oxysterols and its Application in Dextran Sodium Sulphate Induced Mouse Colitis Models
Current Topics in Medicinal Chemistry Development and Applications of Optical Imaging Techniques in Cancer Diagnosis: Diffuse Optical Tomography and Microendoscopy
Current Medical Imaging Safe and Convenient Fluorescent Stain Imager for Proteome Analysis
Current Proteomics Impact of Different Soil Enrichments on the Physicochemical and Antioxidant Potential of Red and White Calyx from Roselle (Hibiscus sabdariffa L.)
Current Nutrition & Food Science Cysteine Network (CYSTEINET) Dysregulation in Parkinson’s Disease: Role of N-acetylcysteine
Current Drug Metabolism New 2-Phenylquinoline Derivatives: Synthesis and Preliminary Evaluation as Antimicrobial Agents
Letters in Organic Chemistry Therapeutic and Protective Potential of Mesenchymal Stem Cells, Pharmaceutical Agents and Current Vaccines Against COVID-19
Current Stem Cell Research & Therapy Microwave-Assisted Synthesis of N-Alkylated Bibenzoimidazolyl Derivatives: Antimicrobial Studies
Letters in Drug Design & Discovery Knee Osteoarthritis and Exercise Adherence: A Review
Current Aging Science Isolation and Characterization of L-Tryptophan Ammonia Lyase from Rubrivivax benzoatilyticus Strain JA2
Current Protein & Peptide Science The Structure and Main Functions of Aminopeptidase N
Current Medicinal Chemistry Imaging Drug Resistance with Radiolabeled Molecules
Current Pharmaceutical Design Analytical Methods for Metallothionein Detection
Current Analytical Chemistry Isoprenoid Metabolism as a Therapeutic Target in Gram-Negative Pathogens
Current Topics in Medicinal Chemistry Comparison of QSARs and Characterization of Structural Basis of Bioactivity Using Partial Order Theory and Formal Concept Analysis: A Case Study with Mutagenicity
Current Computer-Aided Drug Design Synthesis and Evaluation of Vascular Endothelial Growth Factor Receptor-2 Inhibitory Activity of 6,7-Dimethoxycinnoline Derivatives
Letters in Drug Design & Discovery Synthesis and Anti-tubercular Activities of Benzo[4,5]furo[2,3-c] Chromen -6-one Derivatives
Anti-Infective Agents Area, Age and Gender Dependence of the Nucleoside System in the Brain: a Review of Current Literature
Current Topics in Medicinal Chemistry The Antioxidant Properties of Red Sour Cherry (<i>Prunus cerasus</i> L.) Extracts: Laboratory Assessment of Antioxidant Activity and Antioxidant Compounds under Temperature Variations
Current Nutrition & Food Science