Single Cell Proteomics: Challenge for Current Analytical Science
Bo Xu, Wei Du, Bi-Feng Liu and Qingming Luo
Affiliation: The Key Laboratory ofBiomedical Photonics of Ministry of Education, Huazhong University ofScience and Technology (Eastern Campus), Wuhan 430074, P.R. China;
At present, proteomics that promises deep understanding of genomic functions is developing rapidly. Proteomics at single cell level represents new challenges that greatly call for technique innovation in current analytical chemistry. In this mini-review, the concept of single cell proteomics (SCP) is clarified. Recent advancement on strategy and methodology of SCP is reviewed, with emphasis on micro-separation technique improvement such as ultra-thin layer gel electrophoresis, multidimensional micro liquid chromatography and capillary electrophoresis, and microfluidic chip as well. Future development on SCP is also suggested.
Keywords: Single cell proteomics, Protein separation, Chromatography, Capillary electrophoresis, Microfluidic chip, Review
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