NMR Screening Methods in Fragment-Based Drug Discovery
Pp. 67-101 (35)
Consonni Roberto and Veronesi Marina
In the late 90’s, NMR based screening emerged as a powerful technique in the
identification of new targeted molecule in drug discovery, at both industrial and academic levels.
The capacity of finding ligands with low affinity has proved NMR to be a leader technique for
Fragmented Based Drug Discovery (FBDD). This approach, complementary to HTS (High
Throughput Screening), is based on the idea that it is easier to develop small and low affinity
molecules endowed with BEI (Binding Efficiency Index) comparable to potent drug molecules with
respect to the HTS identified molecules. Several NMR screening methods have been developed in
the last 20 years to identify these fragments, and they were generally based on the observation of
protein signals of interest (e.g. SAR by NMR) or on ligand signals (e.g. waterLOGSY). In this
paper, different NMR techniques and their pharmaceutical applications will be summarized and
NMR screening, fragment based drug discovery, 19F-NMR, 1H-NMR, STD, Water-LOGSY, 3-
FABS, FAXS, SAR by NMR, ligand efficiency index (BEI), fragment based approach.
Institute for the Study of Macromolecules (ISMAC), NMR Laboratory, National Council of Research, Milan, Italy.