Frontiers in Natural Product Chemistry

Volume: 1

Indexed in: EBSCO.

“Frontiers in Natural Product Chemistry” is an Ebook series devoted to publishing the latest and most important advances in natural product chemistry. The Ebook series covers all aspects of ...
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Microbial Transformation of Natural Products- A Tool for the Synthesis of Novel Analogues of Bioactive Substances

Pp. 149-162 (14)

Wolfgang Voelter, Roland Wacker, Stanka Stoeva, Rania Tsitsilonis and Christian Betzel

Abstract

Based on isolation, sequence determination and X-ray studies, the primary and three-dimensional structure of the glycoprotein mistletoe lectin I (MLI) are determined. ML-I is constituted of two chains (A chain: 254 amino acid residues; B chain: 264 amino acid residues) linked by a disulfide bridge. Three different structurally identified oligosaccharides (I, II, III) are attached to four Ntype glycosylation sites (NA112, NB61, NB96 and NB136). According to these structural characterizations, ML-I is a member of ribosome inactivating proteins (RIP) of type II. The three-dimensional X-ray structure allows a clear-cut picture of the highly toxic effects of ML-I caused by its RNA-N-glycosidase activity, which is in contrast to its immunomodulating activity, applied for the treatment of cancer patients.

Affiliation:

Abteilung fur Physikalische Biochemie des Physiologisch-chemischen Instituts der Universitat Tubingen, Hoppe-Seyler-Str. 4, 72076 Tubingen, Germany.