Current State of the Art in Large Animal Cloning: Any Lesson?
Pp. 149-155 (7)
Pasqualino Loi and Grazyna Ptak
Following fertilisation, major changes occur in the organisation of chromosomes and genes in the
zygote that contributes to the formation of totipotent cells and their ensuing differentiation to the diverse cell
lineages in the organism. The aim of animal cloning through Somatic Cell Nuclear Transfer (SCNT) is to reestablish
the reprogramming that occurs in the fertilized embryo. SNCT often leads to abnormal development
with a majority of concepti lost before birth. Imprinted genes are amongst the most frequently affected genes, and
their altered expression could in part explain the observed developmental phenotypes. Here, we report our
experience in sheep cloning. We describe the major phenotypic abnormalities observed in the extraembryonic
tissues of sheep clones and suggest some possible strategies to improve SCNT.
Department of Comparative Biomedical Sciences, University of Teramo, Italy.