Background: Due to the appearance of resistant bacterial strains against the antimicrobial
drugs and the reduced efficiency of these valuable resources, the health of a community and the economies
of countries have been threatened. Objective: In this study, the antibacterial assessment of zinc sulfide
nanoparticles (ZnS NPs) against Streptococcus pyogenes and Acinetobacter baumannii has been
Methods: ZnS NPs were synthesized through a co-precipitation method using polyvinylpyrrolidone
(PVP), polyvinyl alcohol (PVA) and polyethylene glycol (PEG-4000). The size and morphology of the
synthesized ZnS NPs were determined by a scanning electron microscope (SEM) and it was found that
the average size of the applied NPs was about 70 nm. In order to evaluate the antibacterial effect of the
synthesized ZnS NPs, various concentrations (50μg/mL, 100 μg/mL and 150 μg/mL) of ZnS NPs were
prepared. Antibacterial assessments were performed through the disc diffusion method in Mueller Hinton
Agar (MHA) culture medium and also the optical density (OD) method was performed by a UV-Vis
spectrophotometer in Trypticase™ Soy Broth (TSB) medium. Then, in order to compare the antibacterial
effects of the applied NPs, several commercial antibiotics including penicillin, amikacin, ceftazidime and
primaxin were used.
Results: The achieved results indicated that the antibacterial effects of ZnS NPs had a direct relation
along with the concentrations and the concentration of 150 μg/mL showed the highest antibacterial effect
in comparison with others. In addition, the ZnS NPs were more effective on Acinetobacter baumannii.
Conclusion: The findings of this research suggest a novel approach against antibiotic resistance.