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Current Biotechnology

Editor-in-Chief

ISSN (Print): 2211-5501
ISSN (Online): 2211-551X

Research Article

Impact of In-vitro Propagation and Organic Farming Cultivation Practices of Artemisia annua L. on the Enhancement of Artemisinin Yield

Author(s): Ankit Agrawal*, Anjana Sharma and Narmada P. Shukla

Volume 9, Issue 1, 2020

Page: [38 - 44] Pages: 7

DOI: 10.2174/2211550109666200306130503

Price: $65

Abstract

Background: Artemisia annua is well known for its anti-malarial bio-active compound artemisinin. Development of elite planting material of A. annua and its agro-technology can fulfill the requirement of Artemisinin-based Combination Therapy (ACT) dosages worldwide.

Objectives: To develop an efficient in-vitro propagation protocol for A. annua and assess the field performance of in-vitro propagated plants for their growth and artemisinin yield.

Methods: The in-vitro propagation protocol of A. annua was developed using the nodal segment in four steps viz: initiation, multiplication, rooting and hardening. In-vitro propagated plants were transplanted with open-pollinated seed raised plants in an experimental field trial having soil supplementation of Farm Yard Manure (FYM), vermicompost and NPK.

Result: Maximum 92% shoots were initiated in Murashige and Skoog medium (MS) with 0.44 μM 6-benzyl aminopurine (BA) and highest 281.33 ± 09.75 micro-shoots/inoculum obtained in MS with 15.54 μM BA. The maximum number of roots was found in MS with 100 mg/L activated charcoal while 78.20% of plants survived in the sand: soil: vermicompost (1:1:1) mixture. The highest dry leaf yield (6.37 t/ha) was observed in in-vitro propagated plants grown with vermicompost, while highest artemisinin content (1.11 ± 0.10) and artemisinin yield (65.05 kg/ha) was found in the in-vitro propagated plants grown with FYM after 120 days of transplantation.

Conclusion: This study reports an efficient, cost-effective and rapid in-vitro propagation protocol for A. annua as well as enhanced artemisinin yield through the cultivation of in-vitro propagated plants using organic soil supplement inputs. This would lead to an increase in the production of artemisinin yield and fulfill the demand of Artemisinin-based Combination Therapy (ACT).

Keywords: Micropropagation, HPTLC, farm yard manure (FYM), vermicompost, agriculture, medicinal plant.

Graphical Abstract
[1]
Anonymous . Chemical studies on qinghaosu (artemisinin): China cooperative group on Qinghaosu and its derivatives as anti-malarials. J Tradit Complement Med 1982; 2: 3-8.
[2]
Duke S, Paul RN. Development and fine structure of the glandular trichomes of Artemisia annua L. Int J Plant Sci 1993; 154: 107-18.
[http://dx.doi.org/10.1086/297096]
[3]
Phillipson JD, Wright CW. Antiprotozoal agents from plant sources. Planta Med 1991; 57: S53-9.
[http://dx.doi.org/10.1055/s-2006-960230]
[4]
WHO World Malaria Report 2018. Geneva: World Health Organization 2018.
[5]
WHO The World Health Report. Geneva: World Health Organization 2001.
[6]
Singh NP, Lai H. Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells. Life Sci 2001; 70(1): 49-56.
[http://dx.doi.org/10.1016/S0024-3205(01)01372-8] [PMID: 11764006]
[7]
Ke OY, Krug EC, Marr JJ, Berens RL. Inhibition of growth of Toxoplasma gondii by qinghaosu and derivatives. Antimicrob Agents Chemother 1990; 34(10): 1961-5.
[http://dx.doi.org/10.1128/AAC.34.10.1961] [PMID: 2291661]
[8]
Merali S, Meshnick SR. Susceptibility of Pneumocystis carinii to artemisinin in vitro. Antimicrob Agents Chemother 1991; 35(6): 1225-7.
[http://dx.doi.org/10.1128/AAC.35.6.1225] [PMID: 1929266]
[9]
Romero MR, Efferth T, Serrano MA, et al. Effect of artemisinin/artesunate as inhibitors of hepatitis B virus production in an “in vitro” replicative system. Antiviral Res 2005; 68(2): 75-83.
[http://dx.doi.org/10.1016/j.antiviral.2005.07.005] [PMID: 16122816]
[10]
Kaptein SJ, Efferth T, Leis M, et al. The anti-malaria drug artesunate inhibits replication of cytomegalovirus in vitro and in vivo. Antiviral Res 2006; 69(2): 60-9.
[http://dx.doi.org/10.1016/j.antiviral.2005.10.003] [PMID: 16325931]
[11]
Noumi E. Traditional medicines for HIV/AIDS and Opportunistic infections in North-West Cameroon: Case of skin infections. Public Health 2011; 1: 44.
[12]
Yadav JS, Babu SR, Sabitha G. Stereoselective total synthesis of (+)-artemisinin. Tetrahedron Lett 2003; 44: 387-9.
[http://dx.doi.org/10.1016/S0040-4039(02)02500-5]
[13]
Paddon CJ, Westfall PJ, Pitera DJ, et al. High-level semi-synthetic production of the potent antimalarial artemisinin. Nature 2013; 496(7446): 528-32.
[http://dx.doi.org/10.1038/nature12051] [PMID: 23575629]
[14]
Peplow M. Synthetic biology’s first malaria drug meets market resistance. Nature 2016; 530(7591): 389-90.
[http://dx.doi.org/10.1038/530390a] [PMID: 26911755]
[15]
Thorpe TA. History of plant tissue culture. Mol Biotechnol 2007; 37(2): 169-80.
[http://dx.doi.org/10.1007/s12033-007-0031-3] [PMID: 17914178]
[16]
Souret FF, Kim Y, Wyslouzil BE, Wobbe KK, Weathers PJ. Scale-up of Artemisia annua L. hairy root cultures produces complex patterns of terpenoid gene expression. Biotechnol Bioeng 2003; 83(6): 653-67.
[http://dx.doi.org/10.1002/bit.10711] [PMID: 12889030]
[17]
Lualon W, De-Eknamkul W, Tanaka H, Shoyama Y, Putalun W. Artemisinin production by shoot regeneration of Artemisia annua L. using thidiazuron. Z Natforsch C J Biosci 2008; 63(1-2): 96-100.
[http://dx.doi.org/10.1515/znc-2008-1-218] [PMID: 18386496]
[18]
Baldi A, Dixit VK. Yield enhancement strategies for artemisinin production by suspension cultures of Artemisia annua. Bioresour Technol 2008; 99(11): 4609-14.
[http://dx.doi.org/10.1016/j.biortech.2007.06.061] [PMID: 17804216]
[19]
Sharma G, Shanker V, Agrawal V. An efficient microprapagation protocol of an elite clone EC-353508 of Artemisia annua L. an important antimalarial plant. Int J Pharma Bio Sci 2011; 2: 205-14.
[20]
Hailu T, Abera B, Mariam EG. In vitro mass propagation of Artemisia (Artemisia annua L.) cv: Anamed. Plant Tissue Cult Biotechnol 2013; 23(2): 165-76.
[http://dx.doi.org/10.3329/ptcb.v23i2.17518]
[21]
Mohammad A, Alam P, Ahmad MM, Ali A, Ahmad J, Abdin MZ. Impact of plant growth regulators (PGRs) on callogenesis and artemisnin content in Artemisia annual. Plants Ind J Biotech 2014; 13: 26-33.
[22]
Wetzstein HY, Porter JA, Janick J, Ferreira JFS, Mutui TM. Selection and clonal propagation of high artemisinin genotypes of Artemisia annua. Front Plant Sci 2018; 9: 358.
[http://dx.doi.org/10.3389/fpls.2018.00358] [PMID: 29636758]
[23]
Georgieva ZE, Atanasov NT, Hristova PD, Konstantinova ZM, Todorova CG. Efficient protocol for mass micropropagation of Artemisia annua L GSC. Biol Pharm Sci 2018; 5(2): 59-68.
[24]
Kumar S, Banerjee S, Dwivedi S, et al. Registration of Jeevanraksha and Suraksha varieties of the anti-malarial medicinal plant Artemisia annua. Curr Res Med Aromat Plants 1999; 21: 47-8.
[25]
Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 1962; 15: 473-97.
[http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x]
[26]
Chandravanshi M, Sahu Y, Agrawal A, Raja W. In vitro Micropropagation of important commorcial medicinal plant: Plumbago zeylanica. Adv Biol Res 2014; 8: 139-42.
[27]
Nik SMM, Keshavarzi MHB, Heidari M, Yarahmadi GR. Studies of changes in chlorophyll content in Artemisia annua L. under the effects of biological and chemical fertilizers. Adv Biol Res (Faisalabad) 2011; 2: 196-202.
[28]
Kumar S, Gupta SK, Singh P, et al. High yields of artemisinin by multi-harvest of Artemisia annua crops. Ind Crops Prod 2004; 19: 77-90.
[http://dx.doi.org/10.1016/j.indcrop.2003.07.003]
[29]
Agrawal A. evaluation and development of simple sequence repeats (ssr) molecular marker for Artemisia annua. PhD dissertation 2013.
[30]
Victorio CP, Lage CLS, Sato A. Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis. Cienc Agron 2012; 43: 539-45.
[http://dx.doi.org/10.1590/S1806-66902012000300017]
[31]
Sairkar P, Chandravanshi MK, Shukla NP, Mehrotra NN. Mass production of an economically important medicinal plant Stevia rebaudiana using in vitro propagation techniques. J Med Plants Res 2009; 3: 266-70.
[32]
Janarthanam B, Rashmi P, Sumathi E. Rapid and efficient plant regeneration from nodal explants of Artemisia annua L. Plant Tissue Cult Biotechnol 2012; 22: 33-9.
[http://dx.doi.org/10.3329/ptcb.v22i1.11257]
[33]
Damtew Z, Tesfaye B, Bisrat D. Leaf, essential oil and artemisinin yield of Artemisia (Artemisia annua L) as influenced by harvesting age and plant population density. World J Agric Sci 2011; 7: 404-12.
[34]
Shiekh AE, Wildung DK, Luby JJ, Sargent KL, Read PE. Long-term effects of propagation by tissue culture or softwood single-node cuttings on growth habit, yield, and berry weight of ‘Northblue’ Blueberry. J Am Soc Hortic Sci 1996; 121: 339-42.
[http://dx.doi.org/10.21273/JASHS.121.2.339]
[35]
Sangoil L. Understanding plant density effects on maize growth and development: An important use to maximize grain yield. Sci Rural 2000; 39: 59-68.
[36]
Mensah DB, Dorcas QM, Kwame BR, Kodjo DS. Comparative study on the field performance of fhia-01 (hybrid dessert banana) propagated from tissue culture and conventional sucker in Ghana. J Plant Dev 2012; 19: 41-6.
[37]
Delabays N, Simonnet X, Gaudin M. The genetics of artemisinin content in Artemisia annua L. and the breeding of high yielding cultivars. Curr Med Chem 2001; 8(15): 1795-801.
[http://dx.doi.org/10.2174/0929867013371635] [PMID: 11772351]
[38]
Malik AA, Suryapani S, Ahmad J, Umar S, Abdin MZ, Mir SR. An attempt to enhance select secondary metabiolites of Artemisia annua L. Pak J Biol Sci 2013; 13: 499-506.
[http://dx.doi.org/10.3923/jbs.2013.499.506]

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