Background: Type 2 Diabetes Mellitus (T2DM) is considered a chronic noncommunicable
disease in which oxidative stress is expected as a result of hyperglycaemia. One of the most recent
approaches is the study of microalgae fatty acids and their possible antioxidant effect.
Objective: This study aimed to analyse the effect of supplementation with n-3 fatty acids extracted
from microalgae on the total antioxidant capacity (TAC) and lipid peroxidation of adipose tissue and
plasma from diabetic (db/db) and healthy (CD1) mice.
Methods: Mice were supplemented with lyophilized n-3 fatty acids extracted from microalgae or
added to the diet, from week 8 to 16. TAC assay and Thiobarbituric Acid Reactive Substances assay
(TBARS) were performed on adipose tissue and plasma samples.
Results: The supplementation of lyophilized n-3 fatty acids from microalgae increased the total antioxidant
capacity in adipose tissue of diabetic mice (615.67μM Trolox equivalents vs 405.02μM
Trolox equivalents from control mice, p<0.01) and in the plasma of healthy mice (1132.97±85.75μM
Trolox equivalents vs 930.64±32μM Trolox equivalents from modified diet mice, p<0.01). There was
no significant effect on lipid peroxidation on both strains.
Conclusion: The use of n-3 fatty acids extracted from microalgae could be a useful strategy to improve
total antioxidant capacity in T2DM.