Detecting Protein-Protein Interaction Based on Protein Fragment Complementation Assay

(E-pub Ahead of Print)

Author(s): Tianwen Wang*, Ningning Yang, Chen Liang, Hongjv Xu, Yafei An, Sha Xiao, Mengyuan Zheng, Lu Liu, Gaozhan Wang, Lei Nie.

Journal Name: Current Protein & Peptide Science

Become EABM
Become Reviewer

Abstract:

Proteins are the most critical executive molecules by responding to the instructions stored in the genetic materials in any form of life. More frequently, proteins do their jobs by acting as a roleplayer that interacts with other protein(s), which is more evident when the function of a protein is examined in the real context of a cell. Identifying the interactions between (or amongst) proteins is very crucial for the biochemistry investigation of an individual protein and for the attempts aiming to draw a holo-picture for the interacting members at the scale of proteomics ( or protein-protein interactions mapping). Here, we introduced the currently available reporting systems that can be used to probe the interaction between candidate protein pairs based on the fragment complementation of some particular proteins. Emphases were put on the principles and details of experimental design. These systems are dihydrofolate reductase (DHFR), β-lactamase, tobacco etch virus (TEV) protease, luciferase, β-galactosidase, GAL4, horseradish peroxidase (HRP), focal adhesion kinase (FAK), green fluorescent protein (GFP), and ubiquitin.

Keywords: protein-protein interaction, protein complementation assay, interaction reporting system, enzyme, fluorescent protein, ubiquitin

Rights & PermissionsPrintExport Cite as

Article Details

(E-pub Ahead of Print)
DOI: 10.2174/1389203721666200213102829
Price: $95

Article Metrics

PDF: 4