Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate

(E-pub Ahead of Print)

Author(s): Laleh Samini, Maryam Khoubnasabjafari, Mohamad M. Alimorad, Vahid Jouyban-Gharamaleki, Hak-Kim Chan, Abolghasem Jouyban*.

Journal Name: Current Analytical Chemistry

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Background: Analysis of drug concentrations in biological fluids is required in clinical sciences for various purposes. Among other biological samples, exhaled breath condensate (EBC) is a potential sample for follow up of drug concentrations.

Methods: A dispersive liquid-liquid microextraction (DLLME) procedure followed by a validated liquid chromatography method was employed for the determination of budesonide (BDS) in EBC samples collected using a homemade setup. EBC is a non-invasive biological sample with possible applications for monitoring drug concentrations. The proposed analytical method is validated according to the FDA guidelines using EBC-spiked samples. Its applicability is tested on EBC samples collected from healthy volunteers receiving a single puff of BDS.

Results: The best DLLME conditions involved the use of methanol (1 mL) as a disperser solvent, chloroform (200 μL) as an extraction solvent, and centrifugation rate of 3500 rpm for 5 minutes. The method was validated over a concentration range of 21-210 μg·L-1 in EBC. Inter- and intra-day precisions were less than 10% where the acceptable levels are less than 20%. The validated method was successfully applied for the determination of BDS in EBC samples.

Conclusion: The findings of this study indicate that the developed method can be used for the extraction and quantification of BDS in EBC samples using a low cost method.

Keywords: Budesonide, Microextraction, Exhaled breath condensate, HPLC-UV, Determination

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Article Details

(E-pub Ahead of Print)
DOI: 10.2174/1573411015666191203104522
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