Login Register Cart 0
Generic placeholder image

Current Pharmaceutical Biotechnology

Editor-in-Chief

ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Study of Anti-oxidant, Anti-inflammatory, Genotoxicity, and Antimicrobial Activities and Analysis of Different Constituents found in Rhizome Essential Oil of Curcuma caesia Roxb., Collected from North East India

Author(s): Manabi Paw, Roktim Gogoi, Neelav Sarma, Sudin K. Pandey, Angana Borah, Twahira Begum and Mohan Lal*

Volume 21, Issue 5, 2020

Page: [403 - 413] Pages: 11

DOI: 10.2174/1389201020666191118121609

Price: $65

Abstract

Background: This investigation was designed to evaluate the chemical composition, antioxidant, anti-inflammatory, genotoxicity, and antimicrobial activities of Curcuma caesia Roxb rhizome essential oil.

Methods: Gas Chromatography/Mass Spectroscopy (GC/MS) analysis was performed to determine the chemical composition, standard antioxidative test DPPH assay, reducing power assay, in vitro antiinflammatory activity (egg albumin denaturation, protease inhibitory assay) by using standard methods. Similarly, antimicrobial activity was tested using the disc diffusion method, minimum inhibitory concentration ability (MIC); while to test genotoxicity, Allium cepa assay was used.

Results: GC/MS analysis revealed eucalyptol (28.55%), epicurzerenone (19.62%), and camphor (21.73%) as the major components of C. caesia rhizome essential oil. Potent antioxidant (IC50= 48.08±0.003 μg/mL), anti-inflammatory (IC50= 121.7±0.0013 μg/mL), and antimicrobial activities of the essential oil were recorded better than the standard drugs Fluconazole for fungus and Ciprofloxacin for bacteria. The essential oil also possessed a strong antibacterial effect against two tested bacterial strains B. subtilis and B. cereus with 7.5 μg/mL MIC value, while for fungal strains the essential oil was most effective against S. cereviaceae with an MIC value of 2.5 μg/mL. All the data were recorded in triplicates. Allium cepa assay revealed minor genotoxicity with mitotic index, MI= 27.70%; chromosome aberration, A= 1.1% of C. caesia rhizome essential oil.

Conclusion: C. caesia rhizome essential oil possesses potent antioxidant, anti-inflammatory, and antimicrobial properties with negligible genotoxicity. Hence, the present study is highly significant for the utilization of rhizome of C. caesia, a high-value ethnopharmacological plant for advanced R & D and commercial application.

Keywords: Curcuma caesia, gas chromatography/mass spectroscopy, antioxidant, anti-inflammatory, genotoxicity, antimicrobial.

« Previous Next »
Graphical Abstract

[1]
Revathy, S.; Elumalai, S.; Merina, B.; Benny, A. Isolation, purification and identification of curcuminoids from turmeric (Curcuma longa L.) by column chromatography. J. Ex. Sci., 2011, 2, 21-25.
[2]
Baghel, S.S.; Baghel, R.S.; Sharma, K.; Sikarwar, I. Pharmacological activities of Curcuma caesia. I.J. GP, 2013, 1-5
[http://dx.doi.org/10.4103/0973-8258.111590]
[3]
Paliwal, P.; Pancholi, S.S.; Patel, R.K. Pharmacognostic parameters for evaluation of the rhizomes of Curcuma caesia. J. Adv. Pharm. Technol. Res., 2011, 2(1), 56-61.
[http://dx.doi.org/10.4103/2231-4040.79811] [PMID: 22171294]
[4]
Hadem, I.K.H.; Majaw, T.; Kharbuli, B.; Sharma, R. Beneficial effects of dietary restriction in aging brain. J. Chem. Neuroanat., 2017, 95, 123-133.
[http://dx.doi.org/10.1016/j.jchemneu.2017.10.001] [PMID: 29031555]
[5]
Sirirugsa, P.; Larsen, K.; Maknoi, C. The genus Curcuma. (Zingiberaceae): Distribution and classification with reference to species diversity in Thailand. Gard. Bull., (Singapore), 2007, 59, 203-220.
[6]
Gurdip, S.; Singh, O.P.; Maurya, S. Chemical and biocidal investigations on essential oils of some Indian Curcuma species. Prog. Crystal Growth and Charct., 2002, 45, 75-81.
[http://dx.doi.org/10.1016/S0960-8974(02)00030-X]
[7]
Borah, A.; Paw, M.; Gogoi, R.; Loying, R.; Sarma, N.; Munda, S.; Lal, M. Chemical composition, antioxidant, anti-inflammatory, anti-microbial and in-vitro cytotoxic efficacy of essential oil of Curcuma caesia Roxb. leaves: An endangered medicinal plant of North East India. Ind. Crops Prod., 2019, 129, 448-454.
[http://dx.doi.org/10.1016/j.indcrop.2018.12.035]
[8]
Araújo, C.C.; Leon, L.L. Biological activities of Curcuma longa L. Mem. Inst. Oswaldo Cruz, 2001, 96(5), 723-728.
[http://dx.doi.org/10.1590/S0074-02762001000500026] [PMID: 11500779]
[9]
Mukunthan, K.S.; Kumar, N.V.A.; Balaji, S.; Trupti, N.P. Analysis of essential oil constituents in rhizome of Curcuma caesia Roxb. from South India. J. Essent.Oil. Bear. Plant., 2014, 17, 647-651.
[10]
Dosoky, N.S.; Setzer, W.N. Chemical composition and biological activities of essential oils of Curcuma species. Nutrients, 2018, 10(9), 10.
[http://dx.doi.org/10.3390/nu10091196] [PMID: 30200410]
[11]
Karmakar, I.; Dolai, N.; Saha, P.; Sarkar, N.; Bala, A.; Haldar, P. K Scavenging activity of Curcuma caesia rhizome against reactive oxygen and nitrogen species. Orient. Pharm. Exp. Med., 2011, 11, 221-228.
[http://dx.doi.org/10.1007/s13596-011-0030-6]
[12]
Das, S.; Mondal, P.; Zaman, M. Curcuma caesia Roxb., and it is medicinal uses: A review. I.J.I.P.C., 2013, 3, 2231-2781.
[13]
Donipati, P.; Sreeramulu, S.H. Preliminary phytochemical screening of Curcuma caesia. Int. J. Curr. Microbiol. Appl. Sci., 2015, 4, 30-34.
[14]
Kagyung, R.; Gjurel, P.R.; Rethy, P.; Singh, B. Ethnomedicinal plant used for gastro intestinal diseasesby Adi tribe of Dehang-Debang biosphere reserve in Arunachal Pradesh. J. Trad. Know., 2010, 9, 496-501.
[15]
Tag, H.; Das, A.K.; Loyi, H. Anti-inflammatory plant used by Khamti tribes of Lohit district in Arunachal Pradesh. Nat. Prod. Radiance, 2007, 4, 334-340.
[16]
Devi, H.P.; Mazumder, P.B.; Devi, L.P. Antioxidant and antimutagenic activity of Curcuma caesia Roxb. rhizome extracts. Toxicol. Rep., 2015, 2, 423-428.
[http://dx.doi.org/10.1016/j.toxrep.2014.12.018] [PMID: 28962377]
[17]
Arulmozhi, D.K.; Sridhar, N.; Veeranjaneyulu, A.; Arora, S.K. Preliminary mechanistic studies on the smooth muscle relaxant effect of hydroalcoholic extract of Curcuma caesia. J. Herb. Pharmacother., 2006, 6(3-4), 117-124.
[http://dx.doi.org/10.1080/J157v06n03_06] [PMID: 17317653]
[18]
Sahu, B.; Kenwat, R.; Chandrakar, S. Medicinal value of Curcuma cassia Roxb: An overview. UKJPB, 2016, 4, 69-74.
[http://dx.doi.org/10.20510/ukjpb/4/i6/134671]
[19]
Kalia, A.N. A text book of industrial pharmacognosy. First Edition. CBS Publishers & Distributors; , 2005, pp. 204-205.
[20]
Silva, M.M.; Lidon, F.C. An overview on applications and side effects of antioxidant food additives. Emir. J. Food Agric., 2016, 28, 823-832.
[http://dx.doi.org/10.9755/ejfa.2016-04-351]
[21]
Diaz, M.N.; Frei, B.; Vita, J.A.; Keaney, J.F., Jr Antioxidants and atherosclerotic heart disease. N. Engl. J. Med., 1997, 337(6), 408-416.
[http://dx.doi.org/10.1056/NEJM199708073370607] [PMID: 9241131]
[22]
Gogoi, R.; Loying, R.; Sarma, N.; Munda, S.; Kumar Pandey, S.; Lal, M. A comparative study on antioxidant, anti-inflammatory, genotoxicity, anti-microbial activities and chemical composition of fruit and leaf essential oils of Litsea cubeba Pers from North-east India. Ind. Crops Prod., 2018, 125, 131-139.
[http://dx.doi.org/10.1016/j.indcrop.2018.08.052]
[23]
Negahban, M.; Moharramipour, S.; Sefidkon, F. Fumigant toxicity of essential oil from Artemisia sieberi Besser against three stored-product insects. J. Stored Prod. Res., 2007, 43, 123-128.
[http://dx.doi.org/10.1016/j.jspr.2006.02.002]
[24]
Zheljazkov, V.; Jekov, D. Heavy metal content in some essential oils and plant extracts. Acta Hortic., 1996, (426), 427-433.
[http://dx.doi.org/10.17660/ActaHortic.1996.426.47]
[25]
Homburger, F.; Boger, E. The carcinogenicity of essential oils, flavors, and spices: a review. Cancer Res., 1968, 28(11), 2372-2374.
[PMID: 4881506]
[26]
Rank, J.; Nielsen, M.H. Genotoxicity testing of wastewater sludge using the Allium cepa anaphase-telophase chromosome aberration assay. Mutat. Res., 1998, 418(2-3), 113-119.
[http://dx.doi.org/10.1016/S1383-5718(98)00118-1] [PMID: 9757013]
[27]
Mahesh, B.; Satish, S. Antimicrobial activity of some important medicinal plant against plant and human pathogens. W.J.A.S., 2008, 4, 839-843.
[28]
Banerjee, A.; Nigam, S.S. Antifungal activity of the essential oil of Curcuma caesia Roxb. Indian J. Med. Res., 1976, 64(9), 1318-1321.
[PMID: 1010624]
[29]
Garg, S.C.; Jain, R.K. Antimicrobial efficacy of essential oil from Curcuma caesia. Int. J. Microbiol., 1998, 38, 169-170.
[30]
Pandey, A.K.; Chowdhury, A.R. Volatile constituents of the rhizome oil of Curcuma caesia Roxb., from central India. Flavour Fragrance J., 2003, 18, 463-465.
[http://dx.doi.org/10.1002/ffj.1255]
[31]
Buddhasukh, D.; Smith, J.; Ternai, B. Essential oil of Curcuma caesia Roxb. Warasan Khana Witthayasat Maha Witthayalai Chiang Mai, 1995, 21, 14-16.
[32]
Mahanta, B.P.; Sut, D.; Kemprai, P.; Paw, M.; Lal, M.; Haldar, S. A 1 H-NMR spectroscopic method for the analysis of thermolabile chemical markers from the essential oil of black turmeric (Curcuma caesia) rhizome: application in post-harvest analysis. Phytochem. Anal., 2019, 31(1), 1-9.
[http://dx.doi.org/10.1002/pca.2863] [PMID: 31243828]
[33]
Adam, K.L.H.; Sen, A. Curcuma Species: A source of anticancer drugs. J. Tumor Med. Prevent., 2017, 5, 1-7.
[34]
Blois, M.S. Antioxidant determination by the use of a stable free radical. Nature, 1958, 181, 1199-2000.
[http://dx.doi.org/10.1038/1811199a0]
[35]
Díaz-Mula, H.M.; Zapata, P.J.; Guillén, F.; Valverde, J.M.; Valero, D.; Serrano, M. Modified atmosphere packaging of yellow and purple plum cultivars. 2. Effect on bioactive compounds and antioxidant activity. Postharvest Biol. Technol., 2011, 61, 110-116.
[http://dx.doi.org/10.1016/j.postharvbio.2011.02.012]
[36]
Sangita, C.; Chatterjee, P.; Dey, P.; Bhattaccharya, S. Evaluation of in-vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pac. J. Trop. Biomed., 2012, 2, 178-180.
[http://dx.doi.org/10.1016/S2221-1691(12)60154-3]
[37]
Kunitz, M. Crystalline soybean trypsin inhibitor: II. general properties. J. Gen. Physiol., 1947, 30(4), 291-310.
[http://dx.doi.org/10.1085/jgp.30.4.291] [PMID: 19873496]
[38]
Babatunde, B.B.; Bakare, A.A. Genotoxicity screening of waste waters from Agbara industrial estate Nigeria evaluated with the Allium test. Pollut. Res., 2006, 25, 227-234.
[39]
Nascimento, G.G.F.; Locatelli, J.; Freitas, P.C.; Silva, G.L. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz. J. Microbiol., 2000, 31
[http://dx.doi.org/10.1590/S1517-83822000000400003]
[40]
Pfaller, M.A.; Messer, S.A.; Coffmann, S. Comparison of visual and spectrophotometric methods of MIC endpoint determinations by using broth microdilution methods to test five antifungal agents, including the new triazole D0870. J. Clin. Microbiol., 1995, 33(5), 1094-1097.
[PMID: 7615712]
[41]
Shahrokh, S.; Emtiazi, G. Toxicity and unusual biological behavior of nanosilver on Gram positive and negative bacteria assayed by microtiter-plate. Euro. J. Biologic. Sci., 2009, 1, 28-31.
[42]
Bhowal, M.; Gopal, M. Eucalyptol: Safety and pharmacological profile. RGUHS J. Pharm. Sci., 2015, 5, 125-131.
[http://dx.doi.org/10.5530/rjps.2015.4.2]
[43]
Figueiredo, A.C.; Barroso, J.G.; Pedro, L.G.; Scheffer, J.J.C. Factors affecting secondary metabolite production in plants: Volatile components and essential oils. Flavour Fragrance J., 2008, 23, 213-226.
[http://dx.doi.org/10.1002/ffj.1875]
[44]
Brown, S.K.; Garver, W.S.; Orlando, R.A. 1,8-cineole: An underappreciated anti-inflammatory therapeutic. J. Biomol. Res. Ther., 2017, 6, 154.
[http://dx.doi.org/10.4172/2167-7956.1000154]
[45]
Krishnaraj, M.; Manibhushanrao, K.; Mathivanan, N. comparative study of phenol content and antioxidant activity between nonconventional Curcuma caesia Roxb. and Curcuma amada Roxb. I.J.P.P., 2010, 4, 1735-8043.
[46]
Mangla, M.; Shuaib, M.; Jain, J.; Kashyap, M. In-vitro evaluation of antioxidant activity of Curcuma caesia Roxb. I.J.P.S.R., 2010, 1, 98-102.
[47]
Angel, G.R.; Vimala, B.; Nambisan, B. Antioxidant and anti-inflammatory activities of proteins isolated from eight Curcuma species. Phytopharmacology, 2013, 4, 96-105.
[48]
Kohli, K.; Ali, J.; Ansari, M.J.; Raheman, J. Curcumin: A natural anti-inflammatory agent. Indian J. Pharmacol., 2005, 37, 141-147.
[http://dx.doi.org/10.4103/0253-7613.16209]
[49]
Grant, W.F. Chromosome aberrations in plants as a monitoring system. Environ. Health Perspect., 1978, 27, 37-43.
[http://dx.doi.org/10.1289/ehp.782737] [PMID: 367773]
[50]
Parveen, Z.; Nawaz, S.; Siddique, S.; Shahzad, K. Composition and antimicrobial activity of the essential oil from leaves of Curcuma longa L. Kasur variety. Indian J. Pharm. Sci., 2013, 75(1), 117-122.
[http://dx.doi.org/10.4103/0250-474X.113544] [PMID: 23901173]
[51]
Hendry, E.R.; Worthington, T.; Conway, B.R.; Lambert, P.A. Antimicrobial efficacy of eucalyptus oil and 1,8-cineole alone and in combination with chlorhexidine digluconate against microorganisms grown in planktonic and biofilm cultures. J. Antimicrob. Chemother., 2009, 64(6), 1219-1225.
[http://dx.doi.org/10.1093/jac/dkp362] [PMID: 19837714]

Rights & Permissions Print Cite
Article Metrics
52
6
© 2024 Bentham Science Publishers | Privacy Policy