The effects of hydrogen-rich water on PI3K/AKT-mediated apoptosis were
studied in rats subjected to myocardial ischemia-reperfusion injury (MIRI).
Sixty rats were divided randomly into a hydrogen-rich water group and a control group.
The hearts were removed and fixed in a Langendorff device. Hearts from the control
group were perfused with K-R solution, and hearts from the hydrogen-rich water group
was perfused with K-R solution + hydrogen-rich water. The two treatment groups were
then divided randomly into pre-ischemic period, ischemic period and reperfusion period
groups(10 rats per group), which were subjected to reverse perfusion for 10 min, normal
treatment for 20 min, and reperfusion for 20 min, respectively. The mRNA and protein
expression levels of PI3K, AKT, p-AKT, FoxO1, Bim and Caspase-3 in each group were
detected by RT-qPCR, immunohistochemistry (IHC) and Western blotting. Caspase-3
activity was detected by spectrophotometry.
Among the hydrogen-rich water group, the PI3K/AKT signaling pathway was significantly
activated, and FoxO1, Bim, and Caspase-3 mRNA and protein levels were significantly
decreased in ischemia-reperfusion subgroup compared with the pre-ischemic and
ischemic subgroups. In the ischemia-reperfusion hydrogen-rich water group, PI3K, AKT
and p-AKT mRNA and protein expression levels were increased while the FoxO1, Bim
and Caspase-3 expression levels were significantly decreased compared with those in
the corresponding control group (P<0.05).
Hydrogen-rich water can activate the PI3K/AKT signaling pathway, alleviate ischemiareperfusion
injury in isolated rat hearts, and inhibit cardiomyocyte apoptosis.