Background: Quinoxaline-1,4-dioxide (QNX) derivatives are synthetic heterocyclic compounds with
multiple biological and pharmacological effects.
Objective: In this study, we investigated the oxidative status of quinoxaline-1,4-dioxides derivatives in modulating
melanoma and glioma cell lines, based on previous results from the research group and their capability to
promote cell damage by the production of Reactive Oxygen Species (ROS).
Methods: Using in vitro cell cultures, the influence of 2-amino-3-cyanoquinoxaline-1,4-dioxide (2A3CQNX), 3-
methyl-2-quinoxalinecarboxamide-1,4-dioxide (3M2QNXC) and 2-hydroxyphenazine-1,4-dioxide (2HF) was
evaluated in metabolic activity, catalase activity, glutathione and 3-nitrotyrosine (3-NT) quantitation by HPLC
in malignant melanocytes (B16-F10, MeWo) and brain tumor cells (GL-261 and BC3H1) submitted to radiotherapy
treatments (total dose of 6 Gy).
Results: 2HF increased the levels of 3-NT in non-irradiated MeWo and glioma cell lines and decreased cell
viability in these cell lines with and without irradiation.
Conclusion: Quinoxaline-1,4-dioxides derivatives modulate the oxidative status in malignant melanocytes and
brain tumor cell lines and exhibited a potential radiosensitizer in vitro action on the tested radioresistant cell