Insulin Resistance in Apolipoprotein M Knockout Mice is Mediated by the Protein Kinase Akt Signaling Pathway

(E-pub Ahead of Print)

Author(s): Shuang Yao, Jun Zhang, Yuxia Zhan, Yuanping Shi, Yang Yu, Lu Zheng, Ning Xu*, Guanghua Luo*.

Journal Name: Endocrine, Metabolic & Immune Disorders - Drug Targets
(Formerly Current Drug Targets - Immune, Endocrine & Metabolic Disorders)

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Background: Previous clinical studies have suggested that apolipoprotein M (apoM) is involved in glucose metabolism and play a causative role in insulin sensitivity.

Objective: We aimed to explore the potential mechanism of apoM on modulating glucose homeostasis and analyze differentially expressed genes by employing ApoM deficient (ApoM-/-) and wild type (WT) mice.

Method: The metabolism of glucose in the hepatic tissues of high-fat diet ApoM-/- and WT mice was measured by a glycomics approach. Bioinformatic analysis was applied for analyzing the levels of differentially expressed mRNAs in the liver tissues of these mice. The insulin sensitivity of ApoM-/- and WT mice was compared using insulin tolerance test and the phosphorylation levels of protein kinase Akt (AKT) following insulin stimulation in different tissues were examined by Western blot.

Results: The majority of the hepatic glucose metabolites exhibited lower concentration levels in the ApoM-/- mice compared with those of the WT mice. Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that ApoM deficiency affected the genes associated with the metabolism of glucose. The insulin tolerance test suggested that insulin sensitivity was impaired in ApoM-/- mice. The phosphorylation levels of AKT in muscle and adipose tissues of ApoM-/- mice were significantly diminished in response to insulin stimulation compared with those noted in WT mice.

Conclusion: ApoM deficiency led to the disorders of glucose metabolism and altered glucose metabolism related genes in mice liver. In vivo data indicated that apoM might augment insulin sensitivity by AKT-dependent mechanism

Keywords: Apolipoprotein M, Glucose metabolism, Microarray, Insulin resistance, Protein kinase Akt phosphorylation, Type 2 diabetes

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(E-pub Ahead of Print)
DOI: 10.2174/1871530319666191023125820

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