Background: Aegilops tauschii is a wild relative of bread wheat. This species has been
reported as the donor of bread wheat D genome. There are also several reports that mentioned the
importance of Ae. tauschii in biotic and abiotic stress tolerance. On the other hands, miRNAs have
been reported as the essential regulatory elements in stress response.
Objective: Therefore, it is important to discover novel miRNAs involved in stress tolerance in this
species. The aim of the current study was to predict novel miRNAs in Ae. tauschii and also
uncover their potential role in stress response.
Method: For this purpose, ESTs, TSAs, and miRBase databases were obtained and used to predict
Results: Our results discovered nine novel stem-loop miRNAs. These predicted miRNAs could be
introduced as the new members of previously identified miRNA families in Ae. tauschii, including
miR156, miR168, miR169, and miR319. The result indicating that miR397 and miR530 are novel
families in this species. Furthermore, several novel stem-loop miRNAs predicted for T. aestivum
showed remarkable similarities to novel Ae. tauschii stem-loops.
Conclusion: Our results demonstrated that predicted novel miRNAs could play a significant role
in stress response.