Role of Whole Plant Extract of Nelumbo nucifera Gaertn in the Treatment of Thrombolysis

Author(s): Charu Sharma, Samrat Chauhan, Sumeet Gupta*, Ashwanti Devi, Anroop Nair.

Journal Name: Cardiovascular & Hematological Agents in Medicinal Chemistry
(Formerly Current Medicinal Chemistry - Cardiovascular & Hematological Agents)

Volume 17 , Issue 2 , 2019

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Abstract:

Aim: This study aims to find out the components responsible for the antithrombotic activity of Nelumbo nucifera.

Material and Methods: Petroleum ether, chloroform and hydroalcoholic extracts of whole plant of Nelumbo nucifera (Lotus) were prepared and assessed for its thrombolytic, anti-platelet aggregation activity and bleeding time. The extracts were further analyzed through HPTLC and GC-MS. Statistical analysis was conducted through ANOVA trailed by Tukey’s multiple comparison test test.

Results: Hydroalcoholic extract showed the highest activity at the concentration of 400μg/ml in thrombolytic assay (42.03 ± 5.76), anti-platelet aggregation assay (57.93 ± 1.68) and bleeding time (70.17 ± 2.16) in comparison to clopodigrel (33.76 ± 3.43), aspirin (66.55 ± 1.86) and aspirin (93.85 ± 2.75) at the concentration of 100 μg/ml respectively. 25 peaks were identified through GC-MS, out of which, ferulic acid (14.2μ/g) and quercetin (5.4 μ/g) are active chemical compounds. HPTLC showed different chromatograms in hydroalcoholic extracts like (1) chlorogenic, (2) quercetin, (3) benzoic acid, (4) caffeic acid, (5) ferulic acid, (6) kaempferol, and (7) gallic acid.

Conclusion: Based on these findings, flavonoids present in hydroalcoholic extract may be developed into a drug for clinical application for the treatment of thrombosis in patients.

Keywords: Bioactive extracts, cardiovascular blood disorders, lotus plant, platelet aggregation assay, qualitative analysis, thrombolysis.

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Article Details

VOLUME: 17
ISSUE: 2
Year: 2019
Page: [115 - 124]
Pages: 10
DOI: 10.2174/1871525717666191016110706

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