Background: Reactive aldehydes are involved in diseases associated with oxidative
stress, including diabetes. Human salivary aldehyde dehydrogenase (hsALDH) presumably protects
us from many toxic ingredient/contaminant aldehydes present in food.
Objective: This study aimed to probe the activity of hsALDH in patients with diabetes and than to
correlate it with various oxidative stress markers in the saliva.
Methods: The saliva samples were collected from total 161 diabetic patients from Rajiv Gandhi
Centre for Diabetes, Jawaharlal Nehru Medical College (JNMC), AMU, Aligarh, (India). HsALDH
activity and markers of oxidative stress [8-hydroxydeoxyguanosine (8-OHDG), malondialdehyde
(MDA) and advanced glycation end products (AGEs)] were measured in the saliva samples.
Results: Patients with early stage of diabetes had higher activity of hsALDH when compared with
the control group. As the history of diabetes increases, the activity of the enzyme decreases and also
higher oxidative stress markers (8-OHDG, MDA and AGEs) are detected in the saliva samples.
Negative significant correlation between hsALDH activity and oxidative stress markers were
observed (p <0.0001).
Conclusion: The activity of hsALDH increases in early stages of diabetes most probably to counter
the increased oxidative stress associated with diabetes. However, in later stages of diabetes, the
activity of the enzyme decreases, possibly due to its inactivation resulting from glycation.