Background: Multi-drug resistance in bacterial pathogens is a major concern of today.
Green synthesis technology is being used to cure infectious diseases.
Objectives: The aim of the current research was to analyze the antibacterial, antioxidant, and phytochemical
screening of green synthesized silver nanoparticles using Ajuga bracteosa.
Methods: Extract of A. bracteosa was prepared by maceration technique. Silver nanoparticles were
synthesized using A. bracteosa extract and were confirmed by UV-Vis spectrophotometer, Scanning
Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). The antibacterial,
anti-biofilm, cell proliferation inhibition, TLC-Bioautography, TLC-Spot screening, antioxidant, and
phytochemical screening were also investigated.
Results: UV-Vis spectrum and Scanning electron microscopy confirmed the synthesis of green nanoparticles
at 400 nm with tube-like structures. FTIR spectrum showed that functional groups of nanoparticles
have a role in capping and stability of AgNP. Agar well diffusion assay represented the maximum
antibacterial effect of ABAgNPs against Escherichia coli, Klebsiella pneumoniae, Streptococcus
pyogenes, Staphylococcus aureus, and Pseudomonas aeruginosa at 0.10 g/mL concentration compared
to ABaqu. Two types of interactions among nanoparticles, aqueous extract, and antibiotics (Synergistic
and additive) were recorded against tested pathogens. Crystal violet, MTT, TLC-bio-autography, and
spot screening supported the findings of the antibacterial assay. Highest antioxidant potential effect in
ABaqu was 14.62% (DPPH) and 13.64% (ABTS) while 4.85% (DPPH) and 4.86% (ABTS) was recorded
in ABAgNPs. Presence of phytochemical constituents showed pharmacological importance.
Conclusion: It was concluded that green synthesis is an innovative technology in which natural products
are conjugated with metallic particles and are used against infectious pathogens. The current research
showed the significant use of green nanoparticles against etiological agents.