Background: MicroRNAs have recently been recognized to be engaged in the development of bone
Objective: This study was performed to elucidate the effects of miR-144-3p on proliferation and osteogenesis of
mesenchymal stem cells (MSCs) from the patients with steroid-associated osteonecrosis (ONFH) and its related
Method: The expression level of miR-144-3p in the MSCs from the proximal femur of the patients was examined
by Real-time PCR. The cell proliferation ability was assayed by MTT. The differentiation ability of MSCs was
assayed by Alizarin Red S (ARS) staining. The interaction between miR-144-3p and frizzled4 (FZD4) was investigated
by Real-time PCR, western blot and luciferase reporter assay.
Results: ONFH samples had the obviously high expression of miR-144-3p compared to the control. MiR-144-3p
had a negative effect on the proliferation and osteogenesis of MSCs. Via targeting FZD4, miR-144-3p decreased
β-catenin nuclear translocation, the transcription of RUNX2 and COL1A1. Over-expression of FZD4 partially
reversed miR-144-3p-induced decrease in the proliferation and osteogenesis of MSCs.
Conclusion: MiR-144-3p might play an important role in the development of ONFH and might be used as a
novel class of therapeutic targets for this disease.