Evaluation of Cytotoxic Activity of Lavandula stoechas Aerial Parts Fractions Against HepG2 Cell Lines

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Author(s): Mohd Aftab Siddiqui, Hefazat Hussain Siddiqui, Anuradha Mishra, Afreen Usmani.

Journal Name: Current Bioactive Compounds

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Abstract:

Background: Lavandula genus is an important member of Labiatae (Lamiaceae) family. People use this medicinal plant in treatment of various diseases around the world. The objective of this work was to to investigate cytotoxic effect of different fractions like ethanol, ethyl acetate and n-hexane from methanol extract of Lavandula stoechas aerial parts on HepG2 cell line and investigation of the chemical composition of the ethanolic fraction of the Lavandula stoechas aerial parts which in study showed cytotoxic effect against HepG2 cell line.

Methods: Methanol extract was prepared by cold maceration. Its fractions were obtained in solvents of increasing polarity, i.e., hexane, ethyl acetate and ethanol. In order to evaluate the cytotoxic effect of Lavandula stoechas MTT assay methods was used. For this purpose, HepG2 cell line were treated with the above mentioned L. stoechas aerial parts fractions with different concentration 10, 25, 50, 100, 250, 500 and 1000 µg/ml. The cells without treatment were served as control.

Results: The result of this study showed that ethanolic fraction showed major reduction in % cell survival in compared to other fractions. These results showed that Lavandula stoechas ethanol fraction was highly inhibitory. Further, phyto chemical analysis of ethanolic fraction was carried out using GC-MS technique and the reports showed Lupeol, Phytol, α-Cadinol, Lup-20(29)-en-3-one, hydrocoumarin, Lup-20(29)-en-3-one. Other compounds included fatty acids and their esters. Some of these compounds are being first time reported here from this plant.

Conclusion: MTT assay showed morphological changes, which clearly supports Lavandula stoecha ethanolic fraction posses anticancer potency may be due to presence of phytosterols.

Keywords: Lavandula stoechas, MTT assay, HepG2 cell line, GCMS analysis, cytotoxic effect

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Article Details

(E-pub Ahead of Print)
DOI: 10.2174/1573407215666190916102325
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