Background: Lectins have been studied in recent years due to their immunomodulatory
Objective: We purified a lectin named OniL from tilapia fish (Oreochromis niloticus) and here we
analyzed the cell proliferation and cytokine production in Balb/c mice splenocytes.
Methods: Cells were stimulated in vitro in 24, 48, 72 hours and 6 days with different concentrations
of OniL and Con A. Evaluation of cell proliferation was performed through [3H]-thymidine
incorporation, cytokines were investigated using ELISA assay and cell viability assay was
performed by investigation of damage through signals of apoptosis and necrosis.
Results: OniL did not promote significant cell death, induced high mitogenic activity in relation to
control and Con A and stimulated the cells to release high IL-2 and IL-6 cytokines.
Conclusion: These findings suggest that, like Con A, OniL lectin can be used as a mitogenic agent
in immunostimulatory assays.