Background: Mushrooms are deemed as a special delicacy in many countries. They are
considered an important cuisine due to their bioactive ingredients and possible health benefits.
Methods: Herein, we measured selected biological properties of methanol extracts of Pleurotus
citrinopileatus and Boletus edulis fruiting bodies including; in vitro antimicrobial activity, anti-α-
glucosidase activity, antioxidant activity, anti-lipase activity and cytotoxic activity against different
cancer cells and normal cells.
Results: B. edulis methanol extracts showed high antimicrobial and anti-α-glucosidase activity. In contrast,
P. citrinopileatus methanol extracts showed superior antioxidant activity indicated by (1,1-
diphenyl-2-picrylhydrazyl) DPPH radical scavenging with half maximal inhibitory concentration of
IC50 37.4 µg/ml, anti-lipase activities with IC50 65.2 µg/ml and high cytotoxicity activity against
HepG2 and HeLa cell lines with IC50 22.8 and 36.7 µg/ml, respectively. Flow cytometric analysis of the
cell cycle was used to show apoptotic effects of methanol extracts against HepG2 and HeLa cells.
Conclusion: P. citrinopileatus and B. edulis methanolic extracts appear to contain biologically active
compounds that might be used to treat some common human diseases.