Background: Cancer is one of the main disease as one of the leading causes of death around the world. New tactics targeting the survival pathways that provide effective, active and affordable drugs are being developed. Purpose; super paramagnetic nanoparticle serves as drug carrier for drug delivery system that has been used for diagnosis and therapy against cancer. Herein, Iron oxide-CMC-TA and Iron oxide-CMC-GA nanoparticles are synthesized for this target. Methods; iron oxide (Fe2O3) nanoparticles are synthesized, bounded to carboxy methyl chitosan (CMC) which are them conjugated to tartaric acid (TA) or gallic acid (GA) to form; Iron oxide-CMC-TA and Iron oxide-CMC-GA nanoparticles. Those nanoparticles were characterized and the cytotoxicity effect was evaluated when associated with/without bee venom to measure the synergetic effect on A549 and WI-38 cell lines. In addition, apoptotic genes expression in A549 was evaluated when treated with both nanoparticles. Results; we showed that the cytotoxicity effect of TA and GA on A549 and WI-38 cell lines was increased when they immobilized on iron oxide-CMC nanoparticles and the effect was synergistically elevated when added to bee venom. The cytotoxic activity of these two nanoparticles was higher in A549 cancer cell line when compared with WI-38 normal cell line. Moreover, the expression of apoptotic genes are elevated. Conclusion, Iron oxide-CMC-TA nanoparticle and Iron oxide-CMC-GA nanoparticle can selectively induce more apoptosis in cancer cell lines than in normal cell lines; which is an important factor in cancer cell targeting process to minimize damage upon normal cells.
Keywords: Bone Morphogenetic Protein, BMP signaling, angiogenesis, vascular development, HHT, Super paramagnetic nanoparticle, gallic acid, bee venom, tartaric acid, cytotoxicity, Gene expression.
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