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ISSN (Print): 2211-5501
ISSN (Online): 2211-551X

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Research Article

Quality Control Assessment of Dhatryadi Ghrita with HPTLC Method

Author(s): Rashmi Saxena Pal* and Amrita Mishra

Volume 8, Issue 2, 2019

Page: [104 - 108] Pages: 5

DOI: 10.2174/2211550108666190902123809

Abstract

Background: Standardization of herbal formulation is mandatory for the quality check of drugs for assurance of their therapeutic value. According to the World Health Organization reports, about 80% of the world’s population relies on traditional medicine. Dhatryadi Ghrita, a polyherbal preparation containing eleven ingredients, has a sweet taste, cold potency and sweet post-digestive effects.

Objective: In this research article, quality control for ghrita has been assessed for some of the ingredients of Dhatryadi Ghrita.

Materials & Methods: Quality control assessment was done with the aid of the HPTLC method. Precoated silica gel GF 254 was used as the stationary phase and the developed method used benzene:ethyl alcohol (9:1v/v). Scanning and quantification of the plates were performed at 254 nm and 366 nm.

Result: The study confirms the presence of different constituents of Ghrita.

Conclusion: The routine analysis of Ghrita was facilitated with simple, accurate and cost-effective HPTLC method. Data has been provided to demonstrate the present and future applicability of the methods of quality control assessment of Dhatryadi Ghrita.

Keywords: Quality control, Dhatryadi Ghrita, HPTLC, Traditional medicine, Emblica officinalis, herbal formulations.

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Graphical Abstract

[1]
Pal RS, Mishra A. Standardization of dhatryadi ghrita: a herbal ghee based ayurvedic medicinal preparation. Open Med J 2018; 5: 47-55.
[http://dx.doi.org/10.2174/1874220301805010047]
[2]
Kajaria D, Tripathi JS, Tiwari SK. Scientific basis for using medicated Ghrita (ghee) in Ayurvedic system of medicine. Ayurpharm Int J Ayur Alli Sci 2013; 2(8): 254-8.
[3]
Shastri A. Vidyotini T of Bhaishajya Ratnavali. 14th edition. Chaukhamba Samskruta Samsthana, Kustharogadhikar, Varanasi 2001.
[4]
Pal RS, Mishra A. A review on Dhatryadi ghrita. Int J Res Ayurveda Pharm 2017; 8(2): 190-5.
[http://dx.doi.org/10.7897/2277-4343.082111]
[5]
Wallis TE. Text Book of Pharmacognosy. 5th ed. New Delhi: CBS Publishers 1985; pp. 571-8.
[6]
Bijauliya RK, Alok S, Kumar M. A comprehensive review on standardization of herbal drugs. Int J Pharm Sci Res 2017; 8(9): 3663-77.
[7]
Arun K, Tapas BT. Shivakumar, Sunil Kumar KN, Arun Raj GR. Standardization of haridradi vati of ayurvedic formulary of India (AFI). Int J Res Ayurveda Pharm 2015; 6(6): 688-91.
[http://dx.doi.org/10.7897/2277-4343.066128]
[8]
Gupta PD, Daswani PG, Birdi TJ. Approaches in fostering quality parameters for medicinal botanicals in the Indian context. Indian J Pharmacol 2014; 46(4): 363-71.
[http://dx.doi.org/10.4103/0253-7613.135946] [PMID: 25097272]
[9]
Arun-Raj GR, Shailaja U, Rao PN, Ajayan S, Thomas NP. Review on the contribution of Ura-Marunnu, a traditional baby care practice in Southern India. Pharma Innovation 2014; 2(11): 42-70.
[10]
Li S, Han Q, Qiao C, Song J, Lung Cheng C, Xu H. Chemical markers for the quality control of herbal medicines: an overview. Chin Med 2008; 3: 7.
[http://dx.doi.org/10.1186/1749-8546-3-7] [PMID: 18588699]
[11]
Mallanava V, Shailaja U, Kumar SKN, Kumar A, Bharati S, Arun-Raj GR. Standardization of karshyahara yoga: An ayurvedic nutraceutical supplement. Int J Res Ayurveda Pharm 2016; 7(1): 94-7.
[http://dx.doi.org/10.7897/2277-4343.07136]
[12]
Kokare NV, Wadkar KA, Kondawar MS. Review on standardization of Herbal churna. Int J Res Ayurveda Pharm 2014; 5(3): 397-401.
[http://dx.doi.org/10.7897/2277-4343.05382]
[13]
Sreejith GS, Ittoop J. Ancheril, Kumar SKN, Anitha MG, Arun-Raj GR. Chemical analysis of Dushivishari agada: An Ayurvedic herbo-mineral formulation to combat residual toxicity. Int J Res Ayurveda Pharm 2016; 7(1): 80-3.
[http://dx.doi.org/10.7897/2277-4343.07133]
[14]
Viswaroopan D, Raj AGR, Shailaja U. Standardization of Ashwagandha Ghrita: A herbal ghee based ayurvedic medicinal preparation. Int J Pharm Sci Res 2016; 7(2): 819-23.
[15]
Waddar S, Rao PN, Gopi KBJ, Arun Raj GR, Waddar S. Standardization of Mulaka (Raphenus sativus) Kshara: a herbal alkaline preparation. J Pharmacogn Phytochem 2014; 3(1): 108-10.
[16]
Rasheed A, Sravya RB, Roja C. A review on standardisation of herbal formulation. Inter J Phytother 2012; 2(2): 74-88.
[17]
Trease GE, Evans WC. “Phenols and phenolic glycosides,” in Textbook of Pharmacognosy vol. 12: 343-83. Balliese, Tindall and Co Publishers, London, UK, 1989
[18]
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 42nd ed.. Pune: NiraliPrakashan 2008; 6.1: p. A1.
[19]
Busse W. The significance of quality for efficacy and safety of herbal medicinal products. Drug Inf J 2000; 34: 15-23.
[http://dx.doi.org/10.1177/009286150003400102]
[20]
Cowen D. Pharmacopoeias and Related Literature in Britain and America, 1618-1847. Aldershot, New Hampshire: Ashgate 2001.
[21]
Eisner S. Guidance for Manufacture and Sale of Bulk Botanical Extracts. Silver Spring, MD: American Herbal Products Association 2001.
[22]
Dhalwal K, Shinde Y. Biradar, Mahadik KR. Simultaneous quantification of bergenin, catechin, and gallic acid from Bergenia ciliata and Bergenia ligulata by using thin-layer chromatography. J Food Compos Anal 2008; 21(6): 496-500.
[http://dx.doi.org/10.1016/j.jfca.2008.02.008]
[23]
Egon Stahl.. 1969 reprint, Thin Layer Chromatography, Springer publica-tion 2008; 21(6): 496-500.
[24]
Wagner H, Baldt S. Plant drug analysis-a Thin Layer Chromatography Atlas 2nd. 1996.
[25]
Heftmann E. Chromatographya laboratory handbook of chromatographic and electrophoretic methods. 3rd. Van Nostarand: Princeton MJ 1975.
[26]
Govil GN, Singh VK. Recent Progress in Medicinal Plants: Standardization of herbal / Ayurvedic formulations. Studium Press LLC Publisher 2009; 24: 46.
[27]
Rasheed A, Reddy SB, Roja C. A review on standardisation of herbal formulation. Intern J Phytother 2012; 2(2): 74-88.
[28]
Organisation Mondiale De La Sante. Quality control methods for medicinal plant materials, 559, (1) Original English. World Health Organisation 1992; p. 159.
[29]
Stahl I. Thin layer chromatography A Laboratory Hand Book. 2nd ed. Berlin: Springer-Verlag 1969; pp. 52-86.
[http://dx.doi.org/10.1007/978-3-642-88488-7_3]
[30]
Sethi PD. High Performance Thin Layer Chromatography. 1st ed. New Delhi: CBS Publishers and Distributors 1996.
[31]
Yadav D, Tiwari N, Gupta MM. Simultaneous quantification of diterpenoids in Premna integrifolia using a validated HPTLC method. J Sep Sci 2011; 34(3): 286-91.
[http://dx.doi.org/10.1002/jssc.201000696] [PMID: 21268251]
[32]
Sen S, Chakraborty R. Revival, modernization and integration of Indian traditional herbal medicine in clinical practice: Importance, challenges and future. J Tradit Complement Med 2016; 7(2): 234-44.
[http://dx.doi.org/10.1016/j.jtcme.2016.05.006] [PMID: 8417092]
[33]
Pan SY, Zhou SF, Gao SH, et al. New Perspectives on how to discover drugs from herbal medicines: Cam’s outstanding contribution to modern therapeutics. Evid Based Complement Alternat Med 2013; 2013627375
[http://dx.doi.org/10.1155/2013/627375] [PMID: 23634172]
[34]
Kumar A. Neelam, Suman S, Saxena R. Physico-analytical study of ghrita formulation indicated in Vataj Artava Dushti with special reference to hypomenorrhoea and oligomenorrhoea. Int J Reprod Contracept Obstet Gynecol 2017; 6: 3960-4.
[http://dx.doi.org/10.18203/2320-1770.ijrcog20174044]
[35]
Lekurwale PS, Pandey K, Yadaiah P. Management of Amavata with ‘Amrita Ghrita’: A clinical study. Ayu 2010; 31(4): 430-5.
[http://dx.doi.org/10.4103/0974-8520.82033] [PMID: 22048534]

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