Standardization of herbal formulation is essential in order to assess the quality of drugs for therapeutic value. According to an estimate of World Health Organization, nearly 80% of population of developing countries relies on traditional medicine. Dhatryadi Ghrita is a polyherbal preparation containing eleven ingredients has Madhura rasa, Sita Veerya and Madhura Vipaka.
In this research paper an attempt has been made to develop standardization method for some of the ingredients of Dhatryadi Ghrita.
Materials & Methods:
Quantitative estimation and stability was done by HPTLC method. Stationary phase used was precoated silica gel GF 254 and mobile phase was benzene:ethyl alcohol (9:1v/v). The plate was scanned and quantified at 254 nm and 366 nm.
The correlation co-efficient of kaempferol was found to be 1.29 % in 6 µl Dhatryadi Ghrita sample. The study confirms the presence of different constituents of Ghrita.
The simple, accurate and cost-effective HPTLC method can be utilized for the routine analysis of quantitative determination of Ghrita. Data has been provided to demonstrate applicability of the methods of standardization of Dhatryadi Ghrita.