Background: Natural product with apoptotic activity could serve as a potential new source
for anti-cancer medicine. Numerous phytochemicals from plants have shown to exert antineoplastic effects
via programmed cell death (apoptosis). Cancer is one of the leading causes of death in prosperous
countries. The subject study was intended to evaluate the anticancer properties of Kalonji extracts
against cancer cell lines HeLa and HepG2 and normal cell lines BHK and VERO were used as normal
Materials & Methods: For the evaluation of anti-proliferative effects, cell viability and cell death in all
groups of cells were evaluated via MTT, crystal violet and trypan blue assays. For the evaluation of
angiogenesis, Immunocytochemistry and ELISA of VEGF were done. Immunocytochemistry and
ELISA of Annexin-V and p53 were performed for the estimation of apoptosis in all groups of cells.
Furthermore, LDH assay, antioxidant enzymes activity (GSH, APOX, CAT and SOD) and RT-PCR
with proliferative and apoptotic markers along with internal control were also performed. Cancer cells
of both cell lines HepG2 and HeLa cells showed reduced viability, angiogenesis and proliferation with
increased apoptosis when treated with Kalonji extracts. Whereas anti-oxidative enzymes show enhanced
levels in treated cancer cells as compared to untreated ones.
Conclusion: It was observed that Kalonji extracts have the ability to induce apoptosis and improve the
antioxidant status of HeLa and HepG2 cells. They can also inhibit the proliferation and angiogenesis in
both these cancer cell lines.