Tubular calyx of flowers of Nyctanthes arbour-tristis contains an apocarotenoid crocin, a major constituent present in saffron stigma. The flowers of N. arbortristis are readily available, hence can be economic substitute for saffron. Lutein from flowers of Tagetes patula, is another carotenoid which is popular antioxidant.
Oxidative stress is a major contributor to the process of aging. Carotenoids are powerful antioxidants.
Hence the study was carried out to evaluate anticollagenase activity and antielastase activity using gene expression study in Human dermal fibroblast.
Crocin was isolated from tubular calyx of Nyctanthes arbortristis using flash chromatographic technique and lutein was isolated using column chromatography. Anticollagenase and antielastase activity of crocin and lutein was carried out using collagenase from Clostridium histolyticum as enzyme and porcine pancreatic elastase. Cytotoxicity of crocin and lutein was determined in Human Dermal Fibroblast Cell Line (HDF) through MTT assay. In gene expression study, HDF Cell line was inoculated with Crocin (450 and 250 ppm) and lutein (100 and 50 ppm) separately for 24 hrs and the m-RNA expression levels of COL Type -1 and elastin were determined using RT-PCR. The results were compared with standards.
Result: The cytotoxic concentration CTC 50 (ppm) for Crocin and lutein was found to be 790.2 ppm and 137.14 ppm.
The cell line studies confirmed that crocin from tubular calyx of Nyctanthes arbortristis and of Tagetes patula have potential to inhibit collagenase as well as elastase enzymes and also a promising agent for upregulation of collagen and elastin gene.